National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Aminoglycosides resistance is a major problem because of their critical role in treating life threatening healthcare associated infections (HCAIs). Therefore, this study aimed to detect aminoglycoside resistant gram-negative bacilli in different clinical isolates in Surgical ICUs of Tanta University Hospital. This study was carried out on 600 patients who had evidence of HCAIs. All specimens were cultured on nutrient, Blood, Mac-Conkey and CLED agars. Out of tested specimens 277 gram-negative bacilli (GNB) isolates were identified by conventional methods of them 100 isolates confirmed by API 20E system and their aminoglycosides resistance was assessed by modified Kirby- Bauer method and E-test. AAC(3)-IIa, AAC(6Õ)-Ib, APH(3′)-VI (APH A6), APH (3′)-I(APH A1), ANT (3”)-Ia (AAD A1) and Arm A genes were detected by conventional (PCR). (39%) of isolates were Acinetobacter baumanii, (28%) Klebsiella pneumoniae, (25%) Pseudomonas aeruginosa, (7%) Proteus mirabilis and (1%) Enterobacter cloacae. All isolates were MDR with 98% of isolates producing ESBL. Over all aminoglycosides resistance were (96%) to tobramycin, (90%) to gentamycin and (89%) to amikacin. AAC(3)-IIa, AAC(6Õ)-Ib, APH A6, APH A1, AAD A1 and Arm A were detected in 22%,70%, 76%,,43%,18%, 43% of the isolates respectively.
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