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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 476-48
DOI: https://doi.org/10.20546/ijcmas.2017.605.055


Evaluation of Endogenous Gene Validation: An Important Contrivance for Differential Gene Study in Pearl Millet
Poonam Kanani* and Y.M. Shukla
Department of Agricultural Biotechnology, Anand Agricultural University, Anand-388 110, India
*Corresponding author
Abstract:

Nowadays selection and validation of endogenous gene is commonly being studied worldwide for differential gene study. And it is also considered as inevitable for gene validation studies. Endogenous gene expression varies profoundly with every cell and tissue with even mere deviations at nominal levels too, thus a need of normalization. So far, the most refined and steadfast method is preferably Quantitative Real-Time PCR (qRT-PCR) for precise quantification of gene expression to understand explicit gene functions at various development phases and beneath diverse environmental conditions that plant encounters during its life. The data normalization to find most stable gene are obtained through different software programs, such as geNorm, Norm finder and Best keeper. Pearl millet [Pennisetum glaucum (L.) R. Br.] a major grain and forage crop, frequently encounter various stresses both biotic and abiotic, and therefore more chances of studying genes responsible for stress adaption. Thus insight into evaluation of endogenous gene (such as actin, tubulin, GAPDH, ubiquitin etc.) validation studies can prove to target more exact molecular mechanism for stress tolerance.


Keywords: qRT-PCR, normalization, gene expression, validation, endogenous gene
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How to cite this article:

Poonam Kanani and Shukla, Y.M. 2017. Evaluation of Endogenous Gene Validation: An Important Contrivance for Differential Gene Study in Pearl MilletInt.J.Curr.Microbiol.App.Sci. 6(5): 476-48. doi: https://doi.org/10.20546/ijcmas.2017.605.055