Nowadays selection and validation of endogenous gene is commonly being studied worldwide for differential gene study. And it is also considered as inevitable for gene validation studies. Endogenous gene expression varies profoundly with every cell and tissue with even mere deviations at nominal levels too, thus a need of normalization. So far, the most refined and steadfast method is preferably Quantitative Real-Time PCR (qRT-PCR) for precise quantification of gene expression to understand explicit gene functions at various development phases and beneath diverse environmental conditions that plant encounters during its life. The data normalization to find most stable gene are obtained through different software programs, such as geNorm, Norm finder and Best keeper. Pearl millet [Pennisetum glaucum (L.) R. Br.] a major grain and forage crop, frequently encounter various stresses both biotic and abiotic, and therefore more chances of studying genes responsible for stress adaption. Thus insight into evaluation of endogenous gene (such as actin, tubulin, GAPDH, ubiquitin etc.) validation studies can prove to target more exact molecular mechanism for stress tolerance.

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