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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 449-460
DOI: https://doi.org/10.20546/ijcmas.2017.605.052


Phenotypic Safety Assessment and Molecular Characterization of Enterococcus feacium MBTU-P1F1 (KF745071) from Infant feces
Anjali Anne Jacob*, T.R. Keerthi and S.S. Resmi
Laboratory of Microbial Biotechnology, School of Biosciences, Mahatma Gandhi University, Kerala, India
*Corresponding author
Abstract:

LAB strain Enterococcus faecium isolated from infant feces was found to show desirable probiotic capabilities. The strain was designated as Enterococcus faecium MBTU-P1F1 and gene accession number was obtained as KF745071. The strain did not have plasmid, it was susceptible to Vancomycin and the Minimum Inhibitory Concentration of Ampicillin towards it was examined. Comparison of the growth profile of Enterococcus faecium MBTU-P1F1 with enteric fever pathogens supported early findings which had proved the higher auto aggregating and adhesion ability of the strain than the pathogens. In vivo studies were carried out in balb/c mice to examine the safety of oral administration of the test strain on the health status of animal model. The study further was focused on the safety of the strain for use as human and animal probiotic at phenotypic level.


Keywords: Enteric fever pathogens, Enterococcus faecium, Phenotypic safety assessment, Probiotic.
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How to cite this article:

Anjali Anne Jacob, T.R. Keerthi and Resmi, S.S. 2017. Phenotypic Safety Assessment and Molecular Characterization of Enterococcus feacium MBTU-P1F1 (KF745071) from Infant fecesInt.J.Curr.Microbiol.App.Sci. 6(5): 449-460. doi: https://doi.org/10.20546/ijcmas.2017.605.052