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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(4): 6-12
DOI: https://doi.org/10.20546/ijcmas.2022.1104.002


Biocatalytic Preparation of Chiral Alcohols with Micro Green Algae: Bio-reduction of Carbonyl Compounds by Chlorogonium Strains as a Novel Biocatalyst
Kohji Ishihara1*, S. Oka1, Y. Hayashi1, Y. Kondo1, Y. Kitagawa1, K. Shimoda2,
Masuoka3 and N. Nakajima4
1Department of Life Science, Facalty of Science, Okayama University of Science, Okayama, Japan
2Department of Biomedical Chemistry, Faculty of Medicine, Oita University, Hasama-machi, Japan
3Department of Research and Development, Institute for Fruit Juice Research in Tsudaka, Co., Ltd., Okayama, Japan
4Department of Nutritional Science, Okayama Prefectural University, Soja, Okayama, Japan
*Corresponding author
Abstract:

Chlorogonium strains were screened to examine the potential ability of microgreen algae to act as biocatalysts. Two culture media (National Institute for Environmental Studies (NIES) recommended AF-6 medium and a synthetic AY medium) were tested for the use in the liquid culture of three algal strains. When static cultured in the AY medium containing acetate and yeast extract under continuous light conditions, these strains showed promising growth. Thus, the stereoselective reduction of various carbonyl compounds using these strains was investigated. This study discovered that these strains could reduce aliphatic and aromatic a-keto esters, a-substituted b-keto ester, and aromatic a-keto amide. Based on the conversion ratio and stereoselectivity of the yielded alcohols, C. elongatum NIES-1357 strains cultured in the AY medium is a potential biocatalyst for the stereoselective reduction of aliphatic a-keto esters, 2-chlorobenzoylformamide, and ethyl 2-methyl-3-oxobutanoate to the corresponding chiral alcohols in the presence of L-alanine.


Keywords: Micro green algae, Chlorogonium, Biocatalyst, Stereoselective reduction
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How to cite this article:

Kohji Ishihara, S. Oka, Y. Hayashi, Y. Kondo, Y. Kitagawa, K. Shimoda, N. Masuoka and Nakajima, N. 2022. Biocatalytic Preparation of Chiral Alcohols with Micro Green Algae: Bio-reduction of Carbonyl Compounds by Chlorogonium Strains as a Novel Biocatalyst.Int.J.Curr.Microbiol.App.Sci. 11(4): 6-12. doi: https://doi.org/10.20546/ijcmas.2022.1104.002