Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 386-394
DOI: https://doi.org/10.20546/ijcmas.2017.605.044


Standardization of Plant Bio-Regulators for In Vitro Shoot Proliferation of Curcuma longa L. Cv. Roma
S. Beura1, A. Sahu2, S. Rout3*, R. Beura4 and P.N. Jagadev5
1Biotechnology-cum-Tissue Culture Centre, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India
2MITS School of Biotechnology, Bhubaneswar-751024, Odisha, India
3College of Forestry, Sam Higginbottom University of Agriculture Technology and Sciences, Allahabad-211007, Uttar Pradesh, India
4College of Basic Science and Humanity, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India
5Dean of Research, Orissa University of Agriculture and Technology,Bhubaneswar-751003, Odisha, India
*Corresponding author
Abstract:

The present investigation was carried out to standardize the plant bio regulators for in vitro shoot proliferation of Curcuma longa L. Cv. Roma. The finger tip of the C. longa was taken as explants and cultured on MS media supplemented with different concentrations of plant bio-regulators. The result showed that the explants surface sterilized with 70% ethanol for 30 Sec, followed by 0.1% HgCl2 for 11 minutes, followed by 1% KCl washing for 1 min reduced the fungal %, bacterial % and death %. The similar treatment recorded highest survival (90%) and aseptic (90%), minimum days to bud emergence (11.31), maximum available days to bud elongation. MS media supplemented with BAP (3.0 mg/l), NAA (0.2 mg/l) and GA3 (0.2 mg/l) produces longer bud (0.7 cm). This protocol can be very useful to the future research worker and as well as entrepreneurs for mass production of Curcuma Longa L. Cv. Roma.


Keywords: Bud, Curcuma longa, explants, Shoot.
Download this article as Download

How to cite this article:

Beura, S., A. Sahu, S. Rout, R. Beura and Jagadev, P.N. 2017. Standardization of Plant Bio-Regulators for In Vitro Shoot Proliferation of Curcuma longa L. Cv. Roma.Int.J.Curr.Microbiol.App.Sci. 6(5): 386-394. doi: https://doi.org/10.20546/ijcmas.2017.605.044