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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(1): 15-19
DOI: https://doi.org/10.20546/ijcmas.2022.1101.003


Evaluate the Four Various Commercial RT-PCR Kits for COVID-19
Bitesh KumariD and Sugandh RathoreiD*
Department of Microbiology, Autonomous State Medical College, Firozabad, U.P., India
*Corresponding author
Abstract:

Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is a emerged human disease during last month of 2019 in china. The ongoing Coronavirus disease-2019, many diagnostic test available (i.e., TrueNat, Rapid, CBNAAT, etc), amongst them Real Time Polymerase Chain Reaction (RT-PCR) a gold standard and most use technique for the diagnosis of COVID-19. Aim: To evaluate the four various commercial COVID-19 RT-PCR diagnostic kits. The prospective observational study conducted in the Department of Microbiology, Autonomous State Medical College, Firozabad, India, from march to August 2021. A total of 85 sample included in this study. Data should be analysis and maintain in Microsoft Excel version 2007. All 85 respiratory sample tested to four RT-PCR kits. Out of 85, 9 (10.6%) and 73 (85.9%) were negative with all kits. 3 were inconclusive with all four kits. A sensitivity of all four kits at least 100% and a specificity of 99%. Conclusion: The assay exhibited that all four kits high specificity and sensitivity for SARS-CoV-2 and good analytical performance using genes and/or virus.


Keywords: Coronavirus Disease-2019, Nucleic acid, Polymerase chain reaction
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How to cite this article:

Bitesh Kumar and Sugandh Rathore. 2022. Evaluate the Four Various Commercial RT-PCR Kits for COVID-19.Int.J.Curr.Microbiol.App.Sci. 11(1): 15-19. doi: https://doi.org/10.20546/ijcmas.2022.1101.003