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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 249-255
DOI: https://doi.org/10.20546/ijcmas.2017.605.030


Screening and Isolation of Protease Producing Bacteria from Rhizospheric Soil of Apple Orchards from Shimla District (HP), India
Kumari Manorma1*, Shweta Sharma2, Himani Singla3, Kirti Kaundal1 and Mohinder Kaur1
1Department of Basic Science (Microbiology), Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan-173230 (HP), India
2Directorate of Mushroom Research, Chambaghat, Solan-173213 (HP), India
3Department of Basic Sciences (Microbiology) UHF, Nauni-Solan, HP, India
*Corresponding author
Abstract:

The objective of present study was to screen and isolate protease producing bacteria from soil samples collected from rhizospheric soil of apple orchard from Kotkhai of Shimla District. Collected soil samples were serially diluted and 0.1ml of sample was spread on skim milk agar plates at 370C for 48 hrs. Out of fifteen, six bacterial colonies from rhizospheric soil samples showed clear zone around the colony indicating protease activity. Among these, two isolates i.e. KK3 and KK4 produced highest protease activity and was identified as Pseudomonas Sp. by physiological, morphological and biochemical test, the isolated protease producing bacteria also having antifungal activity against different plant pathogenic fungi. The above results indicate that these bacterial isolates can be used as a biocontrol agent against different phytopathogens.


Keywords: Protease activity, Rhizosphere soil, Apple orchard, Pseudomonas sp., Skim milk
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How to cite this article:

Kumari Manorma, Shweta Sharma, Himani Singla, Kirti Kaundal and Mohinder Kaur. 2017. Screening and Isolation of Protease Producing Bacteria from Rhizospheric Soil of Apple Orchards from Shimla District (HP), IndiaInt.J.Curr.Microbiol.App.Sci. 6(5): 249-255. doi: https://doi.org/10.20546/ijcmas.2017.605.030