Pre-requisite for any plant biotechnological approach is development of an efficient plant regeneration system in that crop. In this regard the in vitro regeneration was achieved by using cotyledon explants from aseptically raised seedlings of popular Capsicum F1 hybrids Bharat and Indra. Seeds of both hybrids were exposed to different treatments for proper germination, were decontaminated and sown in vitro on half-strength MS medium. The seed soaked in distilled water along with 2 mg/l GA3 for two days prior to sowing had more pronounced effect on both capsicum hybrids recording maximum germination (90.45 and 84.59 %, respectively) in minimum number of days (9.67 days and 10.33 days, respectively). Regeneration potential of cotyledonary explants touching the medium both abaxial and adaxial sides was examined. Tissue culture responses varied with the genotypes, side of explants touching the medium and combinations of growth regulators used. Regeneration per cent (96.30%), number of shoots per explants (4.56) and per cent shoot elongation (82.10%) was maximum in hybrid Indra from cotyledon explant having abaxial side in contact with growing medium supplemented with 7.5 mg/l zeatin along with 2.0 mg/l GA3. When regenerated shoots cultured on MS media supplemented with 0.5 mg/l IBA produced 100% rooting in both the hybrids. The survival percentage of regenerated plantlets during hardening in pots containing sterile mixture of 1:1 coco-peat and vermiculite was higher in Indra (92.31%) as compared to Bharat (85.71%).

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