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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 103-109
DOI: https://doi.org/10.20546/ijcmas.2017.605.013


Molecular Characterization of a Jatropha leaf curl virus (JLCuV) Infecting a New Host Ludwigia parviflora in India
Buddhadeb Roy, Siriya Sultana and Ang Rinzing Sherpa*
Department of Botany, West Bengal State University, Malikapur, Berunanpukuria, 24 North Parganas, Barasat, Kolkata, 700126 (W.B.), India
*Corresponding author
Abstract:

During survey from March 2014 to October 2015, in Barasat, West Bengal, India, incidence of symptoms including leaf curling, leaf yellowing and stunted growth suggestive of virus infection was observed in Ludwigia parviflora and Jatropha gossipiifolia. The occurrence of Jatropha leaf curl virus (JLCuV) was confirmed by symptomatology, southern blot analysis, polymerase chain reaction (PCR) and sequence analysis of the whole genome. Host range study was done in Capsicum annuum and further the transmission of the virus was confirmed by PCR. Partial sequences from L. parviflora and J. gossipiifolia showed that the virus from both the plants shares 89 % with Jatropha leaf curl virus (JLCuV) and 88 % with Jatropha mosaic Indian virus (JMIV) at nucleotide level. The sequence identity between viruses from the two plants shares 99 % at nucleotide level. Further, the whole genome sequence analysis showed that the virus from L. parviflora share 95 % identity with other available sequences of Jatropha leaf curl virus (JLCuV) isolates and shares 77 %-82 % identity with other begomoviruses at nucleotide level. This is the first molecular evidence of Jatropha leaf curl virus infecting Ludwigia parviflora in West Bengal, India.


Keywords: Begomovirus, PCR, Southern blot, Detection, Ludwigia, India.
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How to cite this article:

Buddhadeb Roy, Siriya Sultana and Ang Rinzing Sherpa. 2017. Molecular Characterization of a Jatropha leaf curl virus (JLCuV) Infecting a New Host Ludwigia parviflora in IndiaInt.J.Curr.Microbiol.App.Sci. 6(5): 103-109. doi: https://doi.org/10.20546/ijcmas.2017.605.013