National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study conducted to examine the clinical bovine mastitis to isolate and identify the bacteria causing the disease and to determine the sensitivity pattern of isolates to commonly using antibiotic in Eldamazine locality, Blue Nile State. A total of 45 dairy cows clinically infected by different types of mastitis. Mastitis milk samples were collected from the animals and transported to microbiology laboratory for bacteriological examination. The samples were cultured in blood agar, MacConkey agar and purified in nutrient agar. Gram stain was used for morphological characteristics. Biochemical tests were done to all isolates. Sensitivity test was applied to gentamycin, ampicillin, vancomycin, ciprofloxacin, tetracycline and erythromycin. There were 3 types of mastitis were detected and these were acute mastitis with high prevalence (62.2%) followed by chronic mastitis (35.6%) and gangrenous mastitis (2.2%). The isolates were Staphylococcus spp (73.36%), Streptococcus spp (4.4%), Bacillus spp (8.9%) Pseudomonas spp, and Escherichia spp (8.9%). Also, the results revealed that high prevalence of Staphylococcus epidermidis (8.9%), followed by Staphylococcus hyicus (6.7%), Streptococcus agalactia (4.4%), and Pseudomonas aeruginosa (4.4%). Most effective antibiotics to isolated bacteria were ciprofloxacin, gentamycin, tetracycline, vancomycin and ofloxacin, while resistant to ampicillin and erythromycin. The species Pseudomonas aeruginosa were resistant to all antibiotics used. In conclusion, mastitis is associated with huge economic loss to farmers in the study area, and most effective control is prevention by using good management practices.
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