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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 23-28
DOI: https://doi.org/10.20546/ijcmas.2017.605.003


Effect of Astaxanthin Supplementation on Semen (Karan Fries Bulls) Storage at 5 °C
Simson Soren*, Sohan Vir Singh and Sunil Kumar
Animal Physiology Division, Climate Resilient Livestock Research Centre, Indian Council of Agricultural Research-National Dairy Research Institute (ICAR-NDRI), Karnal (Haryana)-132001, India
*Corresponding author
Abstract:

Astaxanthin (AX) is a xanthophyll carotenoid having the powerful antioxidant ability with much beneficial health effects. The present study was conducted to observe the effect of AX on sperm quality of Karan Fries (Tharparkar X Holstein Friesian) bulls during storage at 5 °C. The neat semen was diluted with Tris egg yolk citric acid fructose (TEYCAF) extender and equally divided into two parts, i.e. control and AX supplementation. The percentage of progressive motility at 0, 24, 48 and 72 hours were 78.75 ± 1.25 vs.78.75 ± 1.25; 77.50 ± 1.44 vs. 72.5 ± 2.5; 77.50 ± 1.44 vs. 71.25 ± 1.25 and 68.75 ± 1.25 vs. 66.25 ± 2.39 in AX supplemented vs. control, respectively. The percentage of live spermatozoa at 0, 24, 48 and 72 hours were as 88.69 ± 0.09 vs. 88.12 ± 1.32; 85.93 ± 0.59 vs. 83.76 ± 0.61; 82.95 ± 1.31 vs. 78.57 ± 0.70 and 80.92 ± 1.26 vs. 74.07 ± 0.95 in AX supplemented vs. control, respectively. The concentration of catalase (CAT) enzyme in supernatant was reduced (p<0.05) in AX supplemented sample at 48 (7.38 ± 1.43 vs.14.62 ± 2.03) and 72 (14.43 ± 1.29 vs.19.45 ± 2.82) hours. The superoxidase dismutase (SOD) concentration was lowered (p<0.05) only at 24 hours of preservation in AX supplemented samples than control. It can be concluded from the study that supplementation of AX to the semen extender assisted in maintaining or protecting the spermatozoa from damage during storage at 5 °C.


Keywords: Astaxanthin, Semen, Storage, Refrigerator temperature.
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How to cite this article:

Simson Soren, Sohan Vir Singh and Sunil Kumar. 2017. Effect of Astaxanthin Supplementation on Semen (Karan Fries Bulls) Storage at 5 °CInt.J.Curr.Microbiol.App.Sci. 6(5): 23-28. doi: https://doi.org/10.20546/ijcmas.2017.605.003