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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1-15
DOI: https://doi.org/10.20546/ijcmas.2017.605.001


SeNPs/PLGA Inhibits Biofilm Formation by Cultivable Oral Bacteria Isolated from Caries-Active Children
Adel El-Tarras1,3, Yousef Al Thomali2, Bahig El-Deeb4 and Hesham El-hariry4,5*
1Biotechnology and Genetic Engineering Unit, Scientific Research Deanship,Taif University, KSA
2Department of Orthodontics and Dentofacial Orthopedics, Faculty of Dentistry,
Taif University, KSA
3Department of Genetics, Faculty of Agriculture, Cairo University, Egypt
4Department of Biology, Faculty of Science, Taif University, KSA
5Department of Food Science, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
*Corresponding author
Abstract:

Total of 523 bacterial isolates were collected from studied children groups (7-10 years); caries-active (n=50), caries-free (n=50). From caries-active children, 325 isolates have belonged to 22 bacterial species, whereas, in caries-free children, 198 bacterial isolates have belonged to 18 species. All isolated bacterial species were fit in 6 different bacterial genera included Actinomyces, Lactobacillus, Porphyromonas, Prevotella, Streptococcus and Veillonella. The predominant bacteria were Streptococcus mutans(96%) and Str. orali(56%) in caries-active children. In caries-free children, the most frequently detected species included Lactobacillus acidophilus (72%) and Str. oralis (36%). Partial sequence of the 16S rRNA gene was determined for confirming the identification of the 22 strong-biofilm bacterial isolates. The minimum inhibitory concentration (MIC90) of Selenium nanoparticles (SeNPs) was 25 µg/mL against most of strong-biofilm bacteria. Sub-MIC90 (25 µg/mL) of SeNPs were incorporated in Poly lactic-co-glycolic acid (PLGA) to fabricate coating surface. PLGA/SeNPs coating surface showed potential reduction effect against biofilm formation by the 22 strong-biofilm strains. In conclusion, SeNPs can be used as a promising agent for effectively preventing biofilm formation by strong-biofilm bacteria related to dental caries.


Keywords: Dental caries, Selenium nanoparticles, PLGA, Antibacterial, Antibiofilm
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How to cite this article:

Adel El-Tarras, Yousef Al Thomali, Bahig El-Deeb and Hesham El-hariry. 2017. SeNPs/PLGA Inhibits Biofilm Formation by Cultivable Oral Bacteria Isolated from Caries-Active ChildrenInt.J.Curr.Microbiol.App.Sci. 6(5): 1-15. doi: https://doi.org/10.20546/ijcmas.2017.605.001