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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:8, August, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(8): 448-455
DOI: https://doi.org/10.20546/ijcmas.2021.1008.055


Microbiological Profile of Chronic Suppurative Otits Media and their Antibiogram in A Rural Tertiary Care Hospital
D. C. Shwetha1*, G. C. Ravi2, D. Venkatesha1, S. Megha1 and P. G. Greeshma2
1Department of Microbiology,
2Department of ENT, AIMS, B G Nagara, Mandya-571448, India
*Corresponding author
Abstract:

Chronic suppurative otitis media (CSOM) is one of the major causes of deafness in India. The chronicity of the disease and poor response to routine antimicrobials prompted us to isolate and identify the causative organisms and study antimicrobial susceptibility pattern. The ear discharge was collected using two sterile cotton swabs. One swab was used for performing Gram’s stain and KOH mount. The second swab was used to inoculate Blood agar, MacConkey agar and Sabouraud Dextrose agar (SDA) for the isolation of aerobic bacteria and fungal pathogens. The antibiotic susceptibility testing of the bacterial isolates was done by Kirby Bauer’s Disc Diffusion method according to CLSI Guidelines. Among 104 samples included in the study, Single bacterial growth was obtained in 69 (66.3%) samples and single fungal growth was seen in 24 (23.1%) samples. Mixed growth was seen in 6 (5.8%) samples and no growth was observed in 5 (4.8%) samples. Among the bacteria Pseudomonas aeruginosa (30.5%) was the predominant isolate and Aspergillus niger (8.6%) was the predominant isolate among fungi. Majority of the Gram negative isolates were sensitive to imipenem and all the Staphylococci isolates were sensitive to vancomycin, teicoplanin and linezolid. Periodic monitoring of microbiological profile is essential for the effective management of CSOM cases in a particular geographical area.


Keywords: Chronic suppurative otitis media, Sabouraud Dextrose agar, antibiotic susceptibility testing, Pseudomonas aeruginosa

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How to cite this article:

Shwetha, D. C., G. C. Ravi, D. Venkatesha, S. Megha and Greeshma, P. G. 2021. Microbiological Profile of Chronic Suppurative Otits Media and their Antibiogram in A Rural Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 10(8): 448-455. doi: https://doi.org/10.20546/ijcmas.2021.1008.055
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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