National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Horsegram is a drought hardy, medicinal, nutritional legume and capable to tolerate various biotic and abiotic stresses. The presence of begomovirus was found to be closely associated with the population build-up of its vector i.e. whitefly (Bemisia tabaci) in different accessions of horsegram in the field. The begomoviral associated disease was detected by using Polymerase chain reaction (PCR) with begomovirus specific degenerate primer. The amplified region of 545 bp were sequenced and submitted to GenBank Accession number MK637450.1. Sequence analysis revealed the highest identity 99.63% with Pedilanthus leaf curl virus. The symptoms were matching with the typical begomoviral symptoms like upward leaf curling and yellow patches with stunted growth respectively. A total of 48 accessions of horsegram were tested against of begomoviral leaf curl disease in a randomized complete block design with three replications check. The maximum average disease incidence of leaf curling found to be 54.44% (L1).100% germination of seeds was given by L4, L14, L29 and L30 accessions. To the best of our knowledge, this is the first report of leaf curl disease in horsegram by begomovirus in India.
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