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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1111-1123
DOI: https://doi.org/10.20546/ijcmas.2017.604.138


Isolation and Identification of Fungal Communities in Organic and Conventional Soils
Nadia El Abed2*, Ibtissem Ben Salem1, Mohamed Ben Khedher2, Mahmoud M’Hamdi2, and Naima Boughalleb-M’Hamdi1
1Department of Biological Sciences and plant Protection, Plant Pathology Laboratory, University of Sousse, Unité de recherche UR 13AGR03, Institut Supérieur Agronomique de Chott Meriem, 4042, Sousse, Tunisia Institut Supérieur Agronomique de Chott Meriem, 4042, Sousse, Tunisia
2Crop Vegetables Laboratory, University of Sousse, Unité de recherche UR 13AGR03, Institut Supérieur Agronomique de Chott Meriem, 4042, Sousse, Tunisia
*Corresponding author
Abstract:

Mycoflora population from soil of organic and conventional fields was investigated at different plots. The fungi were isolated by using soil dilution method. Total of six genera were obtained from 10 soil samples. On the basis of cultural and microscopic characteristics, the isolated strains were identified as Aspergillus sp. Penicillium sp., Alternaria sp., Fusarium sp., Cladosporium sp. and Gliocladium sp. Aspergillus species showed the highest percentage among the six genera. This work aims to search different potential bio-agents fungi from soil's microflora sampled from selective organic plots. In vitro, direct confrontation between pathogens and antagonists showed that Gliocladium roseum, Aspergillus pseudoelegans, A. niger, A. terreus, A. nidulans and A. fumigatus inhibited the mycelial growth of Fusarium solani and Alternaria alternata, with varying efficiencies. The obtained inhibition rates reach up above 44%. The mycoflora analysis proved that these soils are rich in fungi that could play a crucial role in the biological treatment as antagonists of pathogen fungus.


Keywords: Mycoflora, organic, conventional, soil, in vitro, direct confrontation.
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How to cite this article:

Nadia El Abed, Ibtissem Ben Salem, Mohamed Ben Khedher, Mahmoud M’Hamdi, Naima Boughalleb-M’Hamdi. 2017. Isolation and Identification of Fungal Communities in Organic and Conventional Soils.Int.J.Curr.Microbiol.App.Sci. 6(4): 1111-1123. doi: https://doi.org/10.20546/ijcmas.2017.604.138