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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2459-2466
DOI: https://doi.org/10.20546/ijcmas.2017.604.287


Prevalence of Panton Valentine Leukocidin (pvl) Gene in Methicillin Resistant Staphylococcus aureus Isolated from Market Samples of Chicken Meat
Wilfred Ruban1*, R. Narendra Babu2, Robinson J.J. Abraham2, T.M.A. Senthilkumar, P. Kumaraswamy4, V. Appa Rao5
1Department of Livestock Products Technology, Veterinary College, Bangalore, India
2Department of Livestock Products Technology (Meat Science),Madras Veterinary College, Chennai, India
3Department of Animal Biotechnology, Madras Veterinary College, Chennai, India
4Department of Bioinformatics and ARIS cell, Madras Veterinary College, Chennai, India
5Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai, India
*Corresponding author
Abstract:

The present study was conducted to evaluate the prevalence of Panton Valentine Leukocidin (pvl) in methicillin resistant S. aureus isolated from Chicken meat marketed in retail outlets in Chennai city, Tamil Nadu. A total of 120 meat samples were collected from different retail outlets and it was observed that 66.67 (80/120) per cent of the samples were positive for the presence of S. aureus. The isolates were screened for methicillin resistance phenotypically by methicillin, oxacillin and cefoxitin disc diffusion assay and for presence of mecA gene by PCR. The results revealed that 54 isolates were positive for presence of mecA gene by PCR indicating that the prevalence of methicillin resistant S. aureus (MRSA) was 67.5 per cent. Comparison of different disc diffusion assays with mecA PCR revealed that cefoxitin disc diffusion assay has sensitivity, specificity, Positive predictive value (PPV), Negative predicative value (NPV) and accuracy of100, 91, 100, 89 and 95 per cent respectively as compared to oxacillin and methicillin disc diffusion assay.Both MRSA and Methicillin susceptible S. aureus (MSSA) were screened by PCR for the presence of pvl gene and it was observed that 38 isolates carried pvl gene of which 28 isolates were MRSA and 10 isolates were MSSA indicating an overall prevalence of 47.5 per cent (38/80). The results of the present study indicates that MRSA isolated from retail chicken meat carries pvl geneclearly indicating the presence of Community associated-MRSA involving human contamination and hence proper hygiene is essential to prevent possible ill effects to the consumers.


Keywords: S. aureus, Chicken meat, MRSA, mecA gene, pvl gene
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How to cite this article:

Wilfred Ruban, R. Narendra Babu, Robinson J.J. Abraham, T.M.A. Senthilkumar, P. Kumaraswamy, V. Appa Rao and Porteen, K. 2017. Prevalence of Panton Valentine Leukocidin (pvl) Gene in Methicillin Resistant Staphylococcus aureus Isolated from Market Samples of Chicken MeatInt.J.Curr.Microbiol.App.Sci. 6(4): 2459-2466. doi: https://doi.org/10.20546/ijcmas.2017.604.287