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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2433-2442
DOI: https://doi.org/10.20546/ijcmas.2017.604.284


Discrimination of Calophyllum inophyllum L. Progenies Through ISSR Markers
B. Palanikumaran* and K.T. Parthiban
Department of Agroforestry, Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam 641 301, India
*Corresponding author
Abstract:

The current study aims at discriminating the thirty Calophyllum inophyllum progenies thorough the deployment of Inter simple sequence repeats (ISSR) markers. The thirty progenies of Calophyllum inophyllum were collected from the predominant distribution and systematic analysis were carried out to elucidate the genetic divergence at Molecular Biology Lab of Department of Tree Breeding of Forest College and Research Institute, Mettupalayam. Among the ten ISSR markers deployed on the thirty progenies of Calophyllum inophyllum, two ISSR markers viz., UBC823 and UBC834 were found to be polymorphic and the number of bands ranged from eight to eleven. The highest polymorphic information content (PIC) value 0.58 was revealed by the primer UBC823 with the average PIC value of 0.45. Cluster analysis using NTSYS generated dendrogram divided all the 30 progenies into ten distinct groups. The progenies A4 and A7, A5 and A8 were closest at the similarity coefficient of 1.000.The lowest PIC value was observed with the primer UBC 834 (0.33). The present investigation has clearly delineated FCRICI 14 progeny genetically diverse and extends scope for further breeding programme through systematic crossing in order to exploit the heterosis.


Keywords: Calophyllum inophyllum, Genetic diversity, ISSR markers
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How to cite this article:

Palanikumaran, B. and Parthiban, K.T. 2017. Discrimination of Calophyllum inophyllum L. Progenies Through ISSR MarkersInt.J.Curr.Microbiol.App.Sci. 6(4): 2433-2442. doi: https://doi.org/10.20546/ijcmas.2017.604.284