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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
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Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2392-2402
DOI: https://doi.org/10.20546/ijcmas.2017.604.279


In vitro Protocol for Direct Organogenesis in Momordica cymbalaria. Fenzl
T. Devi, V. Rajasree*, V. Premalakshmi, K. Hemaprabha and S. Praneetha
Department of Vegetable Crops, HC & RI, Tamil Nadu Agricultural University, Coimbatore - 641003, Tamil Nadu, India
*Corresponding author
Abstract:

Momordica cymbalaria Fenzl. is a perennial herbaceous climber belongs to cucurbitaceae family and the genus of bitter gourd (Momordica charantia). Fruits are used as vegetables, it possess hypoglycaemic, hypolipidemic, cardio protective, hepatoprotective, nephroprotective and antioxidant properties. It is rich in crude fiber (6.42 g/100 g), calcium (72 g/100g), potassium, sodium and vitamin C (290 g/100g).The major constraints in propagation of Momordica cymbalaria are non-availability of standardized propagation methods and low yield. In vitro propagation technique could be a valuable alternative to conventional methods for mass multiplication. To achieve this goal, investigation on micropropagation of Momordica cymbalaria was carried out at the Tissue Culture Laboratory of Horticultural College and Research Institute, TNAU Coimbatore. For direct organogenesis, MS medium supplemented with 2.0 mgl-1 of BAP and 3.0 mgl-1 of kinetin was found to be the most suitable concentration for maximum shoot regeneration percentage and shoot length for both axillary bud and shoot tip. Efficient rooting was observed in half MS medium fortified with 1.0 mgl-1 IBA+ 0.1 mgl-1 of NAA. Rooted plantlets were hardened with various media among which the sterilized pot mixture, coco peat was found to be the most ideal hardening medium. The present study revealed that micro propagation through direct organogenesis using axillary bud could serve as a viable protocol, when MS medium is augmented with 2.0 mgl-1 BAP is used for shoot regeneration. These shoots multiplied to 7.48 numbers in MS medium supplemented with 3.0 mgl-1 BAP, followed by maximum rooting percentage in half MS medium fortified with 1.0 mgl-1IBA + 0.1mgl-1 of NAA and finally acclimatized in pot mixture, coco peat which afford mass multiplication of Momordica cymbalarias.


Keywords: M. cymbalaria, Micropropagation, Direct organonesis, Shoot regeneration, Rooting, Hardening
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How to cite this article:

Devi, T., V. Rajasree, V. Premalakshmi, K. Hemaprabha and Praneetha, S. 2017. In vitro Protocol for Direct Organogenesis in Momordica cymbalaria. Fenzl.Int.J.Curr.Microbiol.App.Sci. 6(4): 2392-2402. doi: https://doi.org/10.20546/ijcmas.2017.604.279