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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 354-362
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.042


Simultaneous Determination of 17 Amino Acids in Microbial Pigments from Monascus spp By UHPLC Amino Acid Analyser Using Pre-Column Derivatization
R. Vidyalakshmi*, R.Paranthaman and K. Sureshkumar
Department of Food Safety & Quality Testing, (ISO/IEC 17025: 2005, NABL Accredited Lab),  Indian Institute of Crop Processing Technology, Ministry of Food Processing Industries,  Govt. of India, Thanjavur-613 005 (TN), India
*Corresponding author
Abstract:

Microbial pigments were produced from Monascus purpureus (MTCC 410), Monascus purpureus (MTCC 369), Monascus ruber (MTCC 1880) and Monascus ruber (MTCC 2326) by submerged fermentation. The essential amino acids were determined in the pigment extract natural pigments by UHPLC method using precolumn derivatization method with o-phthalaldehyde-2-mercaptoethanol. The results shown in the Monascus purpureus (MTCC 410) produced the mamimum amount of amino acids, viz,  Aspartic Acid ( 5.90 mg/L), Glutamic Acid (1.30 mg/L), Serine (1.80 mg/L),Histldine (0.60 mg/L), Tyrosine (5.70 mg/L), Cystine (0.80 mg/L), Valline ( 2.40 mg/L), Leucine (0.40 mg/L) and Proline (88.20 mg/L). Analysis of these amino acids may be of great importance to determine food safety and consumer safety.


Keywords: Monascus spp, Amino acids, UHPLC, Pre-column derivatization
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How to cite this article:

Vidyalakshmi, R., Paranthaman, R. and Sureshkumar, K. 2016. Simultaneous Determination of 17 Amino Acids in Microbial Pigments from Monascus spp By UHPLC Amino Acid Analyser Using Pre-Column Derivatization.Int.J.Curr.Microbiol.App.Sci. 5(3): 354-362. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.042