Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(4): 470-484
DOI: https://doi.org/10.20546/ijcmas.2021.1004.049


Characterization of Potent Phytate Solubilizing Bacterial Strains of Tea Garden Soils as Futuristic Potent Bio-Inoculant
Swayambhu Ghosh1, Anupjyoti Goswami1, Goutam K. Ghosh2 and Prabhat Pramanik1*
1Department of Soils, Tocklai Tea Research Institute, Tea Research Association,
Jorhat–785008, Assam, India
2Department of Soil Science and Agricultural Chemistry, Palli Siksha Bhavana, Institute of Agriculture, Visva-Bharati, Sriniketan – 731236, West Bengal, India
*Corresponding author
Abstract:

Phosphorus (P) is one of the major essential macronutrients for biological growth and metabolism of plants. Tea (Camellia sinensis L) plantation requires adequate amount of P for the proper yield of tea leaves. However, due to acidic nature of tea growing soils, phosphorus fixation is a common phenomenon. A close look at the different pools of soil P reveals, major portion of soil P is present in the form of organic P. Among various members of organic P, myo-inositol hexakisphosphate (or commonly called as phytate) often dominates in soils. This potent sink of P is unavailable to plants. Only a group of microorganisms can hydrolyze phytate through their phytase enzyme and liberate phosphate, that in turn can contribute to plant nutrition. In this work, 2 phytate solubilizing microorganisms, identified as Bacillus safensis and Bacillus siamensis were isolated from the rhizospheric soils of tea gardens in Assam, India. They were found to show clear halo zone around their colonies on the selective media due to solubilization of Ca-phytate. They were also inoculated in Na-phytate containing Pikovskya’s broth. The phosphate concentration varied from 6.2 to 0.8 µg P ml-1 broth during incubation period. Both of them were able to liberate significantly (p<0.001) higher phosphate in solution through solubilization of phytate present in the media. They were also mass cultured and incubated with tea growing soil under laboratory condition. Both the microbes treated soils showed increase in available P content during the incubation period. Bacillus siamensis treated soil showed a gradual increase in P content till 80 days of the incubation with a value of 16.5±0.8 mg kg-1 soil. This microbe was also found to be increasing significantly higher P in soil than the other microbial strain and control. This work suggests, tea cultivating soils are a natural habitat of phytate solubilizing microorganisms. Also, an indigenous phytate solubilizing microorganism Bacillus siamensis has ability to increase the plant available P in soil. Thus it can be applied in tea garden soils for efficient phosphorus management.


Keywords: Phytate, Phytate solubilizing microorganisms, Tea cultivation, Soil available phosphorus
Download this article as Download

How to cite this article:

Swayambhu Ghosh, Anupjyoti Goswami, Goutam K. Ghosh and Prabhat Pramanik. 2021. Characterization of Potent Phytate Solubilizing Bacterial Strains of Tea Garden Soils as Futuristic Potent Bio-Inoculant.Int.J.Curr.Microbiol.App.Sci. 10(4): 470-484. doi: https://doi.org/10.20546/ijcmas.2021.1004.049