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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:4, April, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(4): 463-469
DOI: https://doi.org/10.20546/ijcmas.2021.1004.048


In vitro Evaluation of Bacterial Bioagents against Fusarium wilt of Banana caused by Fusarium oxysporum f. sp. Cubense
T. C. Archith1*, V. Devappa2 and R. K. Mesta1
1Department of Plant Pathology, College of Horticulture, Bagalkot-587104, Karnataka, India
2Department of Plant Pathology, College of Horticulture, Bengaluru-560065, Karnataka, India
*Corresponding author
Abstract:

The present investigation was undertaken to evaluate potential inhibitory effect of indigenous bacterial biocontrol agents against Fusarium oxysporum f. sp. cubense, a causal organism of wilt disease in Banana crop. Twelve indigenous bacterial bioagents were isolated from banana rhizosphere soil collected from banana growing fields in and around Mysore, Mandya and Chamarajanagar districts, which is geographically located in Southern part of Karnataka, India. In PCR identification, 16S rDNA test, the size of the amplicon was 360 bp and 450 bp in Bacillus spp. and Pseudomonas spp. Amongst six isolates of Pseudomonas spp, and six isolates of Bacillus spp showed significant inhibition against the test pathogen. Among all the Pseudomonas isolates tested, Isolate MPB-2 (Pseudomonas fluorescens) showed maximum inhibition of 38.89 per cent and Bacillus sp CKB-1 showed maximum inhibition of 33.89 per cent. The obtained preliminary results are useful and promising enough for further studies towards isolation and characterization of bacterial bioagents responsible for disease control.


Keywords: Fusarium wilt, Banana, Pseudomonas fluorescens, Bacillus subtilis

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How to cite this article:

Archith, T. C., V. Devappa and Mesta, R. K. 2021. In vitro Evaluation of Bacterial Bioagents against Fusarium wilt of Banana caused by Fusarium oxysporum f. sp. Cubense.Int.J.Curr.Microbiol.App.Sci. 10(4): 463-469. doi: https://doi.org/10.20546/ijcmas.2021.1004.048
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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