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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(4): 386-394
DOI: https://doi.org/10.20546/ijcmas.2021.1004.041


Antibiotic Resistance and Beta-Lactamase Encoding Gene (Blavim) in Pseudomonas aeruginosa Isolated from Clinical Samples from Tanta University Hospitals
Aya G. Abdrabo*, Wesam S. Mohamed, Ghada A. Ahmed and Amany M. Abo El-Enein
Department of Clinical Pathology, Faculty of Medicine, Tanta University, Egypt
*Corresponding author
Abstract:

High mortality rates are often associated with antimicrobial resistance, early detection of these resistant strains and their phenotypic and genotypic confirmation is necessary to decide the suitable therapy. To screen beta-lactamase production and its genes such as bla-VIM in Ps. aeruginosa strains isolated from hospitalized patients in Tanta University Hospitals. The study was carried out on 160 Pseudomonas aeruginosa isolates. Samples had been cultured, identified and tested for sensitivity. A phenotypic test (Double disc synergy test) was applied on the collected MDR isolates for detection of MBL enzyme production. Conventional PCR was performed for the detection of the resistance gene(bla-VIM). 100 isolates were multi-drug resistant according to (Modified Kirby Bauer disc diffusion method), with sensitivity to some drugs specially Amikacin (20%). Phenotypic test for MBL resulted in (38%) positive isolates and (62%) were negative ones. Multiplex PCR detected gene (bla-VIM) in (30%) isolates and it was absent in (70%) of the isolates. High prevalence of MBL among multi-drug resistant P. aeruginosa is thought to be due to the uncontrolled consumption of antimicrobials. Rapid identification of such strains is needed to decrease mortalities.


Keywords: Antibiotic resistance, Pseudomonas aeruginosa, beta-lactamase, Conventional PCR, Bla-VIM
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How to cite this article:

Aya G. Abdrabo, Wesam S. Mohamed, Ghada A. Ahmed and Amany M. Abo El-Enein. 2021. Antibiotic Resistance and Beta-Lactamase Encoding Gene (Blavim) in Pseudomonas aeruginosa Isolated from Clinical Samples from Tanta University Hospitals.Int.J.Curr.Microbiol.App.Sci. 10(4): 386-394. doi: https://doi.org/10.20546/ijcmas.2021.1004.041