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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(4): 180-188
DOI: https://doi.org/10.20546/ijcmas.2021.1004.017


Inhibition Effect of Piper betle L., Piper porphyrophyllum N.E. Br. and Piper aduncum L. Leaf Extract on the Mycelial Growth of Colletotrichum gloeosporioides (Penz.) from a Rubber Tree
Fadli1, Nasril Nasir2, Abdi Dharma1, Syafrizayanti1 and Mai Efdi1*
1Department of Chemistry, 2Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Andalas, KampusLimau Manis, Padang-25163, Indonesia
*Corresponding author
Abstract:

Anthracnose leaf disease in rubber trees, caused by the attack of Colletotrichum gloeosporioides, have led to the serious decline in natural rubber production. Continuous usage of synthetic fungicide leave the toxic residues that harm the surrounding animals and plants and are not environmentally friendly. It needs a better solution with organic products that are safer, more effective and biodegradable. Piper species could be considered as a source of new natural products with potential antifungal activities. Thus, the research aimed to investigate the effect of leaf extracts from Piper betle, P.porphyrophyllum and P.aduncumin inhibiting the growth of C. gloeosporioides from rubber trees in vitro. Of the three Piper species, the crude methanolic extract of P. porphyrophyllum leaves shows the highest growth inhibition percentage (83.62%), followed by P. aduncum L. (79,38 %), P. betle (76.39%). In further examination on the leaf extract of Piper in various solvents, we find that the n-hexane extract of P.aduncumhas the best performance in inhibiting the growth of C. gloeosporioides (GIP of 92.72%); it also beats the performance of Benomyl, a commercial fungicide, at the same concentration. The 50% percentage of growth inhibition (MIC50) of this n-hexane extract towards C. gloeosporioides is reached at a very low concentration, 32 mg/L, while the MIC90 is reached at concentration of 799 mg/mL. The results suggest that the n-hexane leaf extract of P. aduncum is a potential candidate for anti-Colletotrichum agent.


Keywords: Antifungal, Piper betle, Piper porphyrophyllum, Piper aduncum, Colletotrichum gloeosporioides
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How to cite this article:

Fadli, Nasril Nasir, Abdi Dharma, Syafrizayanti and Mai Efdi. 2021. Inhibition Effect of Piper betle L., Piper porphyrophyllum N.E. Br. and Piper aduncum L. Leaf Extract on the Mycelial Growth of Colletotrichum gloeosporioides (Penz.) from a Rubber Tree.Int.J.Curr.Microbiol.App.Sci. 10(4): 180-188. doi: https://doi.org/10.20546/ijcmas.2021.1004.017