National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Surgical site infections (SSI) are the third most commonly reported nosocomial infections. They have been responsible for the increasing cost, morbidity and mortality related to surgical operations. SSI rate has varied from a low of 2.5% to a high of 41.9%. The common organisms encountered in post-operative wound infections are Staphylococcus aureus, Coagulase-negative staphylococci, Escherichia coli, Enterococcus, Proteus, Pseudomonas and Klebsiella species. A working knowledge of the most likely causative organism and the prevailing antibiotic sensitivity/resistance pattern will be of great help to treating physician and patient. The objective of this study was to isolate and identify various gram negative bacilli from surgical site infected cases and determine their antimicrobial susceptibility pattern. Out of 100 culture positive isolates, a total of 66 gram negative bacilli were isolated from infected surgical sites. All isolates were identified as per standard procedures. Antimicrobial susceptibility testing of all isolates was done by Kirby Bauer disc diffusion method as per CLSI guidelines. ESBL production by both double disc synergy test and phenotypic confirmation test recommended by CLSI was performed. Most common gram negative bacilli isolated was P. aeruginosa 31 (26%) followed by K. pneumoniae 10 (8.40%), E. coli 9 (7.56%), C. freundii 6 (5.05%), K. oxytoca 5 (4.20%), Acinetobacter sp. 3 (2.52%) and C. koseri 2 (1.68%). All isolates were sensitive to imipenem. The emergence of Gram-negative bacterial species with acquired resistance to various broad spectrum betalactams is becoming a worldwide clinical problem.
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