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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2244-2255
DOI: https://doi.org/10.20546/ijcmas.2017.604.261


Multidrug Resistant Gram Negative Bacilli Causing Surgical Site Infections: Isolation and Antimicrobial Susceptibility
Ashish Bajaj1* and Sneha Kukanur2
1Department of Microbiology, ESIC Medical College and Hospital, Faridabad, India
2Department of Microbiology, Karwar Institute of Medical Sciences, Karwar, India
*Corresponding author
Abstract:

Surgical site infections (SSI) are the third most commonly reported nosocomial infections. They have been responsible for the increasing cost, morbidity and mortality related to surgical operations. SSI rate has varied from a low of 2.5% to a high of 41.9%. The common organisms encountered in post-operative wound infections are Staphylococcus aureus, Coagulase-negative staphylococci, Escherichia coli, Enterococcus, Proteus, Pseudomonas and Klebsiella species. A working knowledge of the most likely causative organism and the prevailing antibiotic sensitivity/resistance pattern will be of great help to treating physician and patient. The objective of this study was to isolate and identify various gram negative bacilli from surgical site infected cases and determine their antimicrobial susceptibility pattern. Out of 100 culture positive isolates, a total of 66 gram negative bacilli were isolated from infected surgical sites. All isolates were identified as per standard procedures. Antimicrobial susceptibility testing of all isolates was done by Kirby Bauer disc diffusion method as per CLSI guidelines. ESBL production by both double disc synergy test and phenotypic confirmation test recommended by CLSI was performed. Most common gram negative bacilli isolated was P. aeruginosa 31 (26%) followed by K. pneumoniae 10 (8.40%), E. coli 9 (7.56%), C. freundii 6 (5.05%), K. oxytoca 5 (4.20%), Acinetobacter sp. 3 (2.52%) and C. koseri 2 (1.68%). All isolates were sensitive to imipenem. The emergence of Gram-negative bacterial species with acquired resistance to various broad spectrum betalactams is becoming a worldwide clinical problem.


Keywords: Surgical site infection, Extended spectrum beta lactamases, Pseudomonas aeruginosa, post-operative wound infection.
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How to cite this article:

Ashish Bajaj and Sneha Kukanur. 2017. Multidrug Resistant Gram Negative Bacilli Causing Surgical Site Infections: Isolation and Antimicrobial Susceptibility.Int.J.Curr.Microbiol.App.Sci. 6(4): 2244-2255. doi: https://doi.org/10.20546/ijcmas.2017.604.261