National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The growth and productivity of pearl millet are harmfully influenced by an array of biotic and abiotic stresses. Blast is one of them as that cause enormous loss to the yield of pearl millet in India. The present study has been conducted with an objective to identify blast resistant genotypes on the basis of field as well as molecular data generated from gene specific SSR molecular markers. In the current research, a total number of 48 pearl millet genotypes have been screened against blast. In field screening, out of 48 genotypes 5 viz., P343, IP205, THAK1827, THAK0201 and THAK0285 have been found highly resistance, 5 were resistance, 20 moderate resistance , 8 susceptible and 10 highly susceptible. In molecular analysis, 4 germplasm lines viz., IP347, IP383, IP324 and IP351 including varieties, namely: THAK827, THAK0285 and THAK0201 have been found to be resistant against blast. Among the polymorphic SSR markers, the gene diversity varied from 0.5304 to 0.6302 for SSR markers FMBLESTSSR5 and FMBLESTSSR1 respectively with an average of 0.5804 and Polymorphic Information Content (PIC) values ranged between 0.4527 to 0.5620, for the markers FMBLESTSSR5 and FMBLESTSSR1 correspondingly with an average of 0.4975. The major allele frequency varied in range of 0.4792 for primer FMBLESTSSR1 to 0.6042 for primer FMBLESTSSR5 with a mean value of 0.5042. The resistant genotypes identified in this experiment may be utilized as donor of resistance gene against blast to develop improved genotypes which would situate as fence against spread of the disease to newer areas and thus it can boost production and productivity of peal millet.
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