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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 268-276
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.033


Extraction and Bio-chemical Characterization of Protease Enzyme from a Proteolytic bacteria Isolated from Dry Mixed Kitchen Waste
Diptendu Sarkar1* and Goutam Paul2
1New Horizon College of Engineering, Dept. of Biotechnology, Ring Rd, Kadubisanahalli, Bellandur Post, Near Marathalli, Bangalore-560103, India
2Dept. of Physiology, Environmental Physiology Division, Microbiology Unit, University of Kalyani, Nadia, West Bengal-741235, India
*Corresponding author
Abstract:

The present research finding have shown that isolation and biochemical characterization of protease enzyme from proteolytic bacteria isolated from mixed vegetable kitchen waste. Three bacterium were isolated from kitchen waste and were screened for proteolytic enzyme production when grown on protein containing solid media, and designated them as DSP1, DSP2 and DSP3. DSP2 (found as Bacillus sp) was selected based on clearance zones and protease enzyme production was carried out in submerged fermentation. Enzyme production by DSP2 was higher at pH 6.0 and a temperature of 30 °C using sucrose and ammonium sulphate as carbon source and nitrogen source, respectively. The maximal activity of DSP2 protease was at 30 °C, pH 5.0 and was thermostable up to 60 °C. Manganese sulphate had high effect on protease enzyme activity.


Keywords: Submerged Fermentation, Proteolytic Activity, Protease.
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How to cite this article:

Diptendu Sarkar and Goutam Paul. Extraction and Bio-chemical Characterization of Protease Enzyme from a Proteolytic bacteria Isolated from Dry Mixed Kitchen Waste.Int.J.Curr.Microbiol.App.Sci. 5(3): 268-276. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.033