National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present research finding have shown that isolation and biochemical characterization of protease enzyme from proteolytic bacteria isolated from mixed vegetable kitchen waste. Three bacterium were isolated from kitchen waste and were screened for proteolytic enzyme production when grown on protein containing solid media, and designated them as DSP1, DSP2 and DSP3. DSP2 (found as Bacillus sp) was selected based on clearance zones and protease enzyme production was carried out in submerged fermentation. Enzyme production by DSP2 was higher at pH 6.0 and a temperature of 30 °C using sucrose and ammonium sulphate as carbon source and nitrogen source, respectively. The maximal activity of DSP2 protease was at 30 °C, pH 5.0 and was thermostable up to 60 °C. Manganese sulphate had high effect on protease enzyme activity.
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