National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An experiment was carried out to investigate the efficacy of probiotic GalliproTect® and bacitracin methylene disalicylate (BMD) on improving intestinal health against C.perfringens induced necrotic enteritis (NE) in broilers. Five hundred 500 day-old broiler chicks (Cobb 400) were divided into five treatment groups, each with five replicates of 20 chicks each by following a completely randomized design. The treatments were an uninfected control (T1), an infected control (T2), an infected group supplemented with probiotic GalliproTect®at 500 g/tone of feed (T3), containing 2×1010 cfu/g, an infected group supplemented with BMD at 500 g/tone of feed (T4) and an infected group supplemented with probiotic + BMD each at 500 g/tone of feed (T5). Necrotic enteritis was induced in the broilers by oral inoculation of 30,000 sporulated Eimeria necatrix oocysts on day 14 followed by Clostridium perfringens inoculation 1.0 mL (108cfu/mL) on day 19 to 21 in group T3, T4 and T5.On day 28 (7thday post infection) scoring of gross lesions were performed. Illeal mucosal samples were collected for mRNA quantification of TLR and cytokine gene by real time PCR. Histological scores revealed that intestinal necrotic lesions and inflammatory changes were reduced. Toll like receptors (TLRs) and cytokine gene expression revealed that probiotic group found to increase interleukins levels and decrease TLR 2 levels in necrotic enteritis infected chickens. Thus, the results suggested that the probiotic supplementation could able to regulate the intestinal mucosal immune response and there by ameliorate inflammation by altering the cytokine and TLR gene expression.
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