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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2080-2087
DOI: https://doi.org/10.20546/ijcmas.2017.604.245


Microencapsulation of Probiotics by Calcium Alginate and Gelatin and Evaluation of its Survival in Simulated Human Gastro-Intestinal Condition
Smitha Mathews*
Department of Zoology, Assumption College (Autonomous), Chanaganacherry, Kottayam, Kerala, India
*Corresponding author
Abstract:

In the recent past, there has been an explosion of probiotic health-based products. However, there are many reports indicated that there is poor survival of probiotic bacteria in these products. Further, the survival of these bacteria in the human gastro-intestinal system is questionable. Providing probiotic living cells with a physical barrier against adverse environmental conditions is therefore an approach currently receiving considerable interest. Microencapsulation being one of the most modern methods has considerable effects on probiotic survival. In this study Lactobacillus acidophilus (NCDC 014) and Lactobacillus casei (NCDC 018)) were encapsulated using calcium alginate-gelatin and prebiotics (inulin and Lactulose) via extrusion technique, and were incubated in simulated gastric juice (along with pepsin, pH=1.5) for 2 hours and simulated intestinal juice (along with bile salts, pH = 8) for 4 hours at 37oC. . The results indicated that the survival of microencapsulated probiotic increased significantly in simulated gastro-intestinal condition. In general, this study indicated that microencapsulation with alginate-gelatin with prebiotic could successfully and significantly protect L. acidophilus and L. casei against adverse condition of simulated human gastro-intestinal condition and offers an effective means of delivery of viable bacterial cells to the colon.


Keywords: Microencapsulation, Probiotic, Calcium alginate-gelatin, Inulin, Lactulose, Simulated gastro-intestinal condition.
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How to cite this article:

Smitha Mathews. 2017. Microencapsulation of Probiotics by Calcium Alginate and Gelatin and Evaluation of its Survival in Simulated Human Gastro-Intestinal ConditionInt.J.Curr.Microbiol.App.Sci. 6(4): 2080-2087. doi: https://doi.org/10.20546/ijcmas.2017.604.245