National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was implemented to evaluate S. aureus for their 2 major capsular polysaccharide genes namely cap5k and cap8k. Capsule is an important virulence factor of S. aureus which help the organism in its survival by evading host immune system specially phagocytosis. Thirty isolates of S. aureus were obtained from skin wounds of cattle in Bikaner, Rajasthan which were first identified by conventional cultural and biochemical properties and then confirmed by ribotyping based on 23S rRNA gene segment. All the 30 isolates were subjected to capsular typing by duplex PCR targeting cap5K and cap8K genes with specific primers. Capsular typing revealed 9 (30 per cent) isolates with cap5K and 15 (50 per cent) isolates with cap8K gene.
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