National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study was proposed to determine the recovery rate of Aspergillus species in Ankara, Turkey, from soil and their identification as well as to evaluate the proteolytic activity of the isolated strains. 20 different soil samples were collected and searched for the presence of the fungal species after selective plating and internal transcribed spacer (ITS) gene regionsequencing of cPCR fragments. Also it was the first report to screen the biologically active peptides production from the recovered Aspergillus isolates by plate assay method using two agar media including Calcium Caseinate and Skim Milk Agar with different pH values. In addition to the activity of Aspergillus protease as crude extracted enzyme obtained by fermentation was determined using agar well diffusion method. Overall, 9 (45%) samples were morphologically proposed as positive fungal isolates and only six of them (66,7%) were sequenced and identified as Aspergillus nigerwith recovery rate of 30% (6/20). These six A. niger isolates were allocated in the NCBI GenBank Database and assigned GQ229076.1, EU314995.1, GQ229076.1, GQ229076.1, GQ229076.1 and GQ229076.1 accession numbers. The proteolytic activity of the extracted enzyme on Calcium Caseinate Agar of A. nigerisolates was high and showed wide clearance zone of 10, 16 and 20 mm diameter at pH 5, 6.6 and 7.3 respectively in compared to that of a reference A. niger strain ATCC 16404 which was only 8, 12 and 14 mm at same pH.The current proteolytic activity showed qualitative documented results at different PH. Consequently, the acid and alkaline protease activities of A. niger isolates were confirmed. Using Calcium Caseinate and Skim Milk Agar allowed the efficient trial for Aspergillus peptides productionand activity creating large scale production of it.
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