National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Guava which is multinutritive or multifunctional fruit is largely consumed by people. So, there has been substantial interest in the mass production and advancement of elite guava planting materials. Different medium components with variable concentration of plant growth regulators significantly affect regeneration ability either through nodal or shoot tip explants. Recent reports developed the standard protocol using different types of basal medium (MS and WPM) with variable concentration and combination of plant growth regulators for four elite cultivars of guava. Out of variable combination of regulators BAP with either with NAA, IAA and Kinetin showed higher regeneration efficiency. The study showed that four cultivars exhibited high frequency of regeneration that is 115.51% in Hisar Safeda, 110.06% in Hisar Surkha, 175.52% in Lucknow-49 and 125.92% in Allahabad Safeda in nodal explants with WPM medium. Whereas in case of shoot tip explants higher percent was 95.34% in Hisar Safeda, 70.95% in Hisar Surkha, 133.21% in Lucknow and 152.18% in Allahabad Safeda recorded. In the regeneration study, it was observed that combination of BAP (2.5 mg/l), Kinetin (1.0 mg/l) and NAA (0.2 mg/l) with WPM has potential to promote higher regeneration of cultivar Lucknow-49 and Allahabad Safeda. Whereas BAP (2.5 mg/l) with NAA (0.2 mg/l) in Hisar Safeda and BAP (2.5 mg/l) with IAA (0.5 mg/l) in Hisar Surkha showed higher regeneration for both explants. The optimized protocol will be helpful for cultivars specific mass production through in vitro culture.
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