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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 260-267
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.032


Incidence and Molecular Characterization of Extended Spectrum of Beta Lactamase Producing Escherichia coli and Klebsiella pneumoniae isolated from Urinary Tract Infection in Patients with Type 2 Diabetes Mellitus
P. Ajay Kumar1* and C.S. VinodKumar2
1Research Scholar in Microbiology, Bharathiar University, Coimbatore, India
2Department of Microbiology, S. S. Institute of Medical Sciences and Research Centre, Davangere-577005, Karnataka, India
*Corresponding author
Abstract:

Predisposition to urinary tract infections (UTIs) in diabetes mellitus results from several factors. Susceptibility increases with longer duration and greater severity of diabetes. The continuous emergence of resistance to antimicrobial agents among the prevalent pathogens is the most dangerous threat for the treatment of infectious disease in type 2 diabetes mellitus. The production of ß-lactamases is the major mechanism of bacterial resistance to ß-lactam antibiotics which considered the most widely used class of antibiotic. Hence it is necessary to know the prevalence of ESBL so as to formulate a policy of empirical therapy in type 2 diabetes mellitus of the study was to characterization of ESBL genes in E. coli and Klebsiella pneumoniae from urinary tract infection among type 2 diabetic patients. A prospective study was carried out on 1560 diabetic patients with urinary tract infection during the period of July 2011 to June 2015. 277 Gram negative bacterial were isolated and identified by standard laboratory techniques. Antimicrobial sensitivity testing was performed on Mueller-Hinton agar plates with commercially available discs by the Kirby-Bauer disc diffusion method. 90 E.coli isolates and 64 K.pneumoniae were tested for ESBL production and PCR was performed using 5 sets of previous published primers to amplify type-specific ESBL genes, including CTX-M-1, CTX-M-2, CTX-M-9, TEM and SHV 46.7% of E.coli and 59.4% of K.pneumoniae were ESBL producers. Of the 42 ESBL-producing E. coli isolates, 38 (90.5%) were positive for ESBL genes and 47.4 % of the E.coli isolates had at least 2 ESBL genes. and CTX-M-9 was seen in 7 (18.4%) isolates. Among K. pneumoniae, 32 (84.2%) of the 38 isolates were positive for ESBL genes and The number of ESBL- producing K. pneumoniae with TEM was in 6 (15.8%) isolates. This study highlights the high prevalence of ESBL production among E. coli and K. pneumoniae in the urinary tract infection among type 2 diabetic patients. Therefore, genotyping of ESBL is need of the hour for monitoring the significant ESBLs that can lead to treatment catastrophe in diabetic patients.


Keywords: Urinary tract infection, Type 2 diabetes mellitus, Extended spectrum beta  lactamases, TEM, SHV, CTX-M.
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How to cite this article:

Ajay Kumar, P., VinodKumar, C.S. 2016. Incidence and Molecular Characterization of Extended Spectrum of Beta Lactamase Producing Escherichia coli and Klebsiella pneumoniae isolated from Urinary Tract Infection in Patients with Type 2 Diabetes Mellitus.Int.J.Curr.Microbiol.App.Sci. 5(3): 260-267. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.032