National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bisphenol A (BPA) is a known Endocrine disrupting chemical. It affects plant growth by changing the ratio of endogenous growth and stress hormone levels. In the present study, BPA bioremediation was carried out using nanoemulsion based organogel comprising Trametes versicolor (TV) laccase enzyme. The effect of BPA and its bioremediated products was studied on two different plant species, i.e. Wheat (Triticum aestivum) and Chickpea (Cicer arietinum) grown hydroponically. Genotoxicity of BPA was assessed by Allium cepa assay. BPA showed inhibition effect on chickpea in dose dependent manner and completely inhibited its germination at 100 mg/L concentration. It affected growth of wheat and chickpea seedlings in early stages of development at 25 mg/L concentration. There were significant phytotoxic effects caused by BPA in terms of reduction of root and shoot length with respect to both plant species. BPA exposure (25 mg/L) for 21 days resulted in reduction in wet weight and dry weight of seedlings of wheat and chickpea. However, there were no toxic effect TV laccase organogel treated BPA (100 mg/L) was observed in both plant species. The seedling grown hydroponically in treated BPA solution has shown similar growth indices as control (no BPA), which indicates its reduced phytotoxicity. Reduction in mitotic index and chromosomal aberrations in Allium cepa root tip cells suggest genotoxicity of BPA. The bioremediated products of BPA showed no cytotoxic effects indicating elimination of its clastogenic effect on plant cells, that may be due to structural modification of BPA mediated by TV laccase.
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