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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:1, January, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(1): 3535-3544
DOI: https://doi.org/10.20546/ijcmas.2021.1001.417


Screening Technique for Identification of Resistant Genotypes against Post Flowering Stalk Rot Complex caused by Macrophomina phaseolina in Maize (Zea mays L.)
Banoth Madhu, K Prabhavathi, D Bhadru and B Mallaiah
Department of Genetics and Plant Breeding, College of Agriculture, Rajendranagar, Professor Jayashankar Telangana State Agricultural University, Hyderabad-500 030, India
*Corresponding author
Abstract:

In order to screen maize genotypes for resistance to post-flowering stalk rot (PFSR) complex caused by Macrophomina phaseolina under field conditions, toothpick method was used for creating artificial epiphytotics. In this study, 98 maize genotypes (20 parents (15 Lines × 5 testers), 75 Single Cross Hybrids (SCHs) and three standard checks) were screened in field by toothpick method of inoculation. The field screening of maize genotypes by the standard toothpick method which needs about 40 days (only at harvesting stage) for expression of plant drying symptoms due to PFSR and data are possible to record only at the time of crop harvesting. Screening is done by using 1-9 rating scale of PFSR for scoring disease severity in-vivo condition. All these maize inbred lines were screened in field by toothpick method of inoculation at Maize Research Centre, Agricultural Research Institute, Rajendranagar, Hyderabad. As a result, most of the genotypes were exhibited disease reaction varying from resistant (score-2) to moderately resistant (score-5) against M. phaseolina. In ordered to identified PFSR resistant lines, screening of 98 maize genotypes in field against M. phaseolina, only four lines, viz., MGC-237, MGC-248, MGC-254, MGC-256 and two testers, viz., BML-6 and GP-311. Whereas, 15 crosses viz., MGC-9 × BML-6, MGC-9 × BML-14, MGC-32 × BML-14, MGC-32 × GP-170, MGC-92 × GP-170, MGC-137 × GP-311, MGC-237 × BML-7, MGC-242 × BML-14, MGC-248 × GP-311, MGC-252 × BML-14, MGC-252 × GP-311, MGC-254 × BML-14, MGC-254 × GP-311, MGC-256 × GP-170 and MGC-256 × GP-311 were found resistant.


Keywords: Maize genotypes, Lines, Testers, Crosses, Standard checks, Tooth pick method of inoculation, Rating scale, Resistant, Screening, Disease score, Stalk rot complex

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How to cite this article:

Banoth Madhu, K Prabhavathi, D Bhadru and Mallaiah, B 2021. Screening Technique for Identification of Resistant Genotypes against Post Flowering Stalk Rot Complex caused by Macrophomina phaseolina in Maize (Zea mays L.).Int.J.Curr.Microbiol.App.Sci. 10(1): 3535-3544. doi: https://doi.org/10.20546/ijcmas.2021.1001.417
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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