An experiment was conducted on Genetic variability, correlation, path coefficient and genetic diversity D² analysis in the 16 genotypes of Cucumber during 2019-20 at the Horticulture Research Field, Department of Horticulture, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj. The observations were recorded on various growths and yield attributes. The experiment was carried out with 16 genotypes procured from Local market of Prayagraj in randomized Block Design (RBD) with three replications. The results from the present investigation revealed that genotypes Swarn Agati identified as high fruit yield per vine, fruit yield per plot (kg)fruit yield (t ha^-1)at Prayagraj Agro-climatic condition. The genotypic and phenotypic coefficient variance value were categorized as low (0-10%), moderate (10-20%) and high (20% and above). The heritability was categorized as low (0-30%), moderate (30- 60%) and high (60% and above).The Genetic advance and Genetic advance as percent mean was categorized as low (0-10%), moderate (10-20% and (≥20%). Genotypic and phenotypic correlation coefficient analysis revealed that yield (t/ha) showed positive significant association with  Germination %, Vine Length (cm), No. of Secondary Branches, Fruit Length (cm), Fruit Diameter (cm), No. of Fruits per Vine, Avg. Fruit Weight (g), Yield per Vine (kg) and Yield per Plot (kg). Genotypic path coefficient analysis revealed that the direct positive effect on fruit yield (t ha-1) was exhibited by germination %,no. of secondary branches, node number to 1st male flower, days to 1st female flower appearance, fruit length (cm),avg. fruit weight (g),yield per vine (kg)and yield per plot (kg).Phenotypic path coefficient analysis revealed that the direct positive effect on fruit yield (t ha-1) was exhibited by germination %, node number to 1st male flower, days to 1st female flower appearance, number to 1st female flower, fruit diameter (cm, avg. fruit weight (g) and yield per plot (kg).On the other hand cluster I,  comprised 5 genotype, cluster III comprised genotypes1 and cluster IV cluster comprised 3genotype. The inter cluster distance was maximum between cluster II and IV. Cluster I, II, III and IV was characterized by high mean value for vine length and low mean values for yield per vine (kg). The highest contribution in manifestation of genetic divergence was exhibited by Sex Ratio (M: F), Yield per Plot (kg), Fruit Length (cm), Yield (t/ha), Fruit Diameter (cm) and Yield per Vine (kg).

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