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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 247-253
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.030


Isolation of Pseudomonas aeruginosa from various Clinical Isolates and it Antimicrobial Resistance Pattern in a Tertiary Care Hospital
Saroj Golia, Suhani, S. Manasa* and Jyoti
Institute-Dr.B.R.Ambedkar Medical College, K.G.Halli, Bengaluru, India
*Corresponding author
Abstract:

Pseudomonas aeruginosa is an opportunistic human pathogen and is the leading cause of nosocomial infections especially in immune compromised patients. In recent years, a considerable increase in the prevalence of multidrug resistance (MDR) P. aeruginosa has been noticed with high morbidity and mortality, hence requiring antibiotic susceptibility testing on a regular as well as a periodic basis. The present study was undertaken to determine the antibiogram of P. aeruginosa and its frequency of occurence from various clinical samples. A study was undertaken with 120 samples which were taken from patients of Dr. B. R. Ambedkar Medical College and hospital. The study was carried out in the Department of Microbiology, Dr. B.R.A.M.C, K. G. Halli, Bengaluru for a period of 9 months from July 2014- March 2015.A total of 120 clinically significant P. aeruginosa isolates were collected from different clinical samples, and processed using conventional microbiological methods. The strains were cultured and identified by standard microbiological techniques and Kirby- Bauer disc diffusion antibiotic susceptibility testing was done for each. Majority of isolates of P. aeruginosa (102/120, 85%) were obtained from specimens of pus, sputum, ear discharge and tracheal aspirates. Among 112 isolated pathogens 35(31.25%) showed resistance to aminoglycosides, 30(26.78%) to cephotaxime. Resistance rates to Piperacilin/tazobactam, ceftazidime, ofloxacin varied from 10-15(8.92% to 13.39%).10/112(8.92%)isolates were multi-drug resistant. All strains were found to be sensitive to imipenam, colistin (100%). The results confirmed the occurrence of drug resistant strains of P. aeruginosa. Imipenem, colistin, ceftazidime, pipperacillin-tazobactem and cefipime were found to be the most effective antimicrobial drugs. It therefore calls for a very judicious, rational treatment regimens prescription by the physicians to limit the further spread of antimicrobial resistance among the P. aeruginosa strains.


Keywords: aeruginosa, Antimicrobial resistance pattern, Clinical isolates.
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How to cite this article:

Saroj Golia, Suhani, Manasa S. and Jyoti. 2016. Isolation of Pseudomonas aeruginosa from various Clinical Isolates and it Antimicrobial Resistance Pattern in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 5(3): 247-253. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.030