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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1787-1795
DOI: https://doi.org/10.20546/ijcmas.2017.604.214


Isolation, Identification and Molecular Detection of Brucella abortus from Buffaloes in Gujarat, India
Kirit B. Patel, H.C. Chauhan, B.K. Patel, S.S. Patel, M.D. Shrimali, J.K. Kala, S.I. Patel, A.N. Modi, A.C. Patel, Manish Rajgor, M.A. Patel, M.G. Patel and B.S. Chandel
Department of Animal Biotechnology and Microbiology, COVSc and AH, SDAU, Sardarkrushinagar-385006, Gujarat, India
*Corresponding author
Abstract:

Brucellosis is an infectious disease, has a considerable impact on animal health as well as socioeconomic impacts. It causes significant reproductive losses in sexually mature animals. The present study reports the isolation, identification and molecular detection of Brucella abortus. A total of 192 buffalo clinical samples were processed by cultural isolation on BBL Brucella agar plate with selective antibiotic supplements and direct PCR. Out of 192 clinical samples, 7 samples growth yielded on BBL Brucella agar plate and identify Brucella organism by colony character, Gram’s staining, MZN staining, Catalase, KOH and Triple Sugar Iron Agar (TSI) test. Moreover, the confirmation of these isolate as Brucella abortus was carried out by genus specific PCR using B4/B5 (223bp), species specific PCR using +IS711 (498bp), and SYBR green based real time PCR. In direct PCR out of 192 clinical samples, 12 samples detected positive by genus specific PCR using B4/B5 (223bp). Genus specific PCR positive 12 samples conform Brucella abortus by species specific PCR using +IS711 (498bp), and SYBR green based real time PCR. In this study 7 clinical samples positive for B. abortus by culture isolation as well as direct PCR. However, 5 clinical samples positive by direct PCR but could not be growth yielded on BBL Brucella agar plate.


Keywords: Buffalo, Brucella abortus, Molecular detection, PCR, Real time PCR.
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How to cite this article:

Kirit B. Pate, H.C. Chauhan, B.K. Patel, S.S. Patel, M.D. Shrimali, J.K. Kala, S.I. Patel, A.N. Modi, A.C. Patel, Manish Rajgor, M.A. Patel, M.G. Patel and Chandel, B.S. 2017. Isolation, identification and molecular detection of Brucella abortus from buffaloes in Gujarat, IndiaInt.J.Curr.Microbiol.App.Sci. 6(4): 1787-1795. doi: https://doi.org/10.20546/ijcmas.2017.604.214