National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Certain strains of microalgae known as diatoms were shown, under appropriate conditions, to produce up to 60% of their cellular mass as lipids under certain growth conditions. Acetyl-Co-A carboxylase catalyses the first committed step in the synthesis of fatty acids, i.e., carboxylation of acetyl-CoA to malonyl-CoA. In present study we have isolated the two species of marine diatoms Odontella aurita and Chaetoceros muelleri from Vellar estuary Parangipettai Southest coast of India and studied the acetyl co-A carboxylase activity at different Si treatment nutrient medium. Results show that ACCase activity was maximum in treatment T2 (53 µM Si concentration) 0.035±0.006 U/ mg protein in O. aurita, 0.037±0.006 U/ mg protein in C. muelleri and minimum in treatment T4 (153 µM Si concentration) 0.019±0.001 U/ mg protein in O. aurita, 0.023±0.001 U/mg protein in C. muelleri. ACCase activity in treatment (T1) 0.031±0.0012 in O. aurita, 0.032±0.006 U/mg protein in C. muelleri while treatment (T3) show 0.024±0.001 in O. aurita, 0.026±0.001 U/ mg protein in C. muelleri. Treatment T2 show maximum ACCase activity so treatment (T2) further optimised. Optimum pH for ACCase activity was 8.0 and temperature 300C and 0.5 mM acetyl co-A. All treatments, acetyl co-A carboxylase activity slightly higher in C. muelleri than O. aurita.
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