National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Iron is one of the essential mineral for all organisms and its deficiency leads to many serious problems in human being worldwide. Plants have evolved with specific ZIP transporters to uptake and transport the iron from the soil. IRT2 is one of the ZIPs family members, which has a role in uptake of Fe2+ from the soil. Indian barnyard millet (E. frumentacea L.) is one of the rich sources of iron among millets. IRT2 gene of Indian barnyard millet is up regulated under iron deficient condition and hence we isolated EfIRT2 gene. In silico analysis of EfIRT2 gene showed that it codes for 386 amino acids, having molecular weight 40.54 kDa with pI 8.65. Its predicted structure showed that it is a plasma membrane protein with eight conserved domains and a signal peptide towards N-terminal end, with a Histidine signature of ZIPs family in between domain III and IV. Analysis based on deduced amino acid sequences of EfIRT2 showed highest similarity with SiIRT2 gene and clustered together with IRT2 proteins of Poaceae family members. Like other ZIPs family members, EfIRT2 could be a potential candidate gene for biofortification of staple foods.
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