National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Avian reovirus (ARV) is a non-enveloped double stranded RNA virus of poultry and is associated with significant economic losses to the poultry industry throughout the world. The virus is responsible for different clinical manifestations in poultry out of which most important is viral arthritis/tenosynovitis. Protein σB, an outer capsid protein of ARV contains group specific neutralizing epitopes and induces strong immune response in natural infection in chicken. We have evaluated the immunogenicity of full length rσB fusion protein in chicken. Six, 4 week old, SPF chickens (Gr. A) were inoculated with 50 µg of rσB protein emulsified with Freund’s incomplete adjuvant (FIA). Control birds (Gr.B and C) received FIA alone and PBS respectively. Blood samples were collected at 0 d.p.i, 7 d.p.i and 21 d.p.i to evaluate the level of cytokine expression towards rσB. Serum neutralization test (SNT) was performed to analyze antibody response. There was significant up regulation of IFN-γ and TNF-α 7 d.p.i in group A birds. There was significant elevation in (P < 0.05) neutralizing antibody titre up to 6.3 in group A birds on 21 d.p.i, whereas there was no detectable neutralizing antibody response in control birds. There was significant increase in CD4+ Th cell population and reached up to 27.06% on 7 d.p.i. The rσb fusion protein of ARV was able to generate good immune response in birds after primary immunization. This result suggests that rσB fusion protein is a good candidate for preparation of subunit vaccine against ARV infection.
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