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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1496-1507
DOI: https://doi.org/10.20546/ijcmas.2017.604.183


Evaluation of Immune Response to rσB Protein of Avian reovirus (ARV) in Chicken
S. Majumder1, T.K.S. Chauhan2, K. Dhama3, S. Nandi1, P.P. Goswami2 and Deepak Kumar2*
1Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, 243122 (U.P.), India
2Division of Veterinary Biotechnology, Indian Veterinary Research Institute,
Izatnagar, 243122 (U.P.), India
3Avian Disease Section, Indian Veterinary Research Institute, Izatnagar, 243122 (U.P.), India
*Corresponding author
Abstract:

Avian reovirus (ARV) is a non-enveloped double stranded RNA virus of poultry and is associated with significant economic losses to the poultry industry throughout the world. The virus is responsible for different clinical manifestations in poultry out of which most important is viral arthritis/tenosynovitis. Protein σB, an outer capsid protein of ARV contains group specific neutralizing epitopes and induces strong immune response in natural infection in chicken. We have evaluated the immunogenicity of full length rσB fusion protein in chicken. Six, 4 week old, SPF chickens (Gr. A) were inoculated with 50 µg of rσB protein emulsified with Freund’s incomplete adjuvant (FIA). Control birds (Gr.B and C) received FIA alone and PBS respectively. Blood samples were collected at 0 d.p.i, 7 d.p.i and 21 d.p.i to evaluate the level of cytokine expression towards rσB. Serum neutralization test (SNT) was performed to analyze antibody response. There was significant up regulation of IFN-γ and TNF-α 7 d.p.i in group A birds. There was significant elevation in (P < 0.05) neutralizing antibody titre up to 6.3 in group A birds on 21 d.p.i, whereas there was no detectable neutralizing antibody response in control birds. There was significant increase in CD4+ Th cell population and reached up to 27.06% on 7 d.p.i. The rσb fusion protein of ARV was able to generate good immune response in birds after primary immunization. This result suggests that rσB fusion protein is a good candidate for preparation of subunit vaccine against ARV infection.


Keywords: Avian reovirus, Sigma B protein.
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How to cite this article:

Majumder, S., T.K.S. Chauhan, K. Dhama, S. Nandi, P.P. Goswami and Deepak Kumar. 2017. Evaluation of immune response to rσB protein of Avian reovirus (ARV) in chickenInt.J.Curr.Microbiol.App.Sci. 6(4): 1496-1507. doi: https://doi.org/10.20546/ijcmas.2017.604.183