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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 3142-3150
DOI: https://doi.org/10.20546/ijcmas.2020.912.374


Isolation of Bacteria from Onions with Brown Rot Symptoms in Enugu State, Nigeria
Mbah-Omeje Kelechi1* and Chukwu Ebuka Joseph2
1Department of Microbiology, Enugu State University of Science and Technology, Agbani, Enugu State, Nigeria
2Renaissance University, Ogboka, Enugu State, Nigeria
*Corresponding author
Abstract:

The study was conducted to investigate bacteria associated with the spoilage of onion bulbs vended in parts of Enugu State, Nigeria. A                 total of 20 samples were collected from Enugu main market. Samples were cultured on nutrient agar and isolates identified using standard methods based on their microbial and biochemical characteristics. Nutritional and proximate analysis was carried out using standard methods of AOAC, (2010). The bacteria species isolated were Bacillus subtilis, Shigella spp, and Escherichia coli. Escherichia coli had the highest occurrence at (15)75% while Shigella spp occurred lowest at (5)25%. The proximate analysis showed high amount of moisture content at 73.99 ± 0.14aa while ash content had the least at 0.26±0.30aa. Mineral Composition showed high amount of potassium at 67.90mg/kg while lead and copper occurred least at 0.01mg/kg and 0.18 mg/kg respectively. The pathogenicity test on the onions showed that Bacillus subtilis and Escherichia coli were the causes of spoilage on onions. There is need to develop new acceptable pre-harvest and post-harvest disinfection methods.


Keywords: Nutritional and Proximate analysis, Pathogenicity, Isolates
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How to cite this article:

Mbah-Omeje Kelechi, N. and Chukwu Ebuka Joseph. 2019. Isolation of Bacteria from Onions with Brown Rot Symptoms in Enugu State, Nigeria.Int.J.Curr.Microbiol.App.Sci. 9(12): 3142-3150. doi: https://doi.org/10.20546/ijcmas.2020.912.374