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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1423-1430
DOI: https://doi.org/10.20546/ijcmas.2017.604.174


Prevalence of ESBL and MBL Producing Gram Negative Isolates from Various Clinical Samples in a Tertiary Care Hospital
Narinder Kaur1*, Amandeep Kaur2 and Satnam Singh3
1Department of Microbiology, Adesh Medical College and Hospital, Ambala, India
2Department of Microbiology, 3Department of Pharmacology,Adesh institute of Medical Sciences and Research, Bathinda, India
*Corresponding author
Abstract:

Antimicrobial resistance is a growing threat worldwide. Increasing resistance to third generation cephalosporins has become a cause for concern. The prevalence of extended spectrum–lactamases (ESBLs) and metallobetalactamases (MBL) among members of Enterobacteriaceae constitutes a serious threat to current -lactam therapy leading to treatment failure. A total of 276 Gram negative isolates were processed. ESBL was detected by phenotypic confirmatory disc diffusion test (PCDDT) using ceftazidime alone and in combination with clavulanic acid. MBL detection was done by Imipenem EDTA combined disc diffusion test and Modified Hodge test. Out of 276 isolates 69 (25%) were ESBL producers, 70 (25.3%) were MBL producers and 26 (9.42%) were both ESBL and MBL producers. Highest ESBL production was seen in Escherichia coli (46%) and maximum MBL production was seen in Klebsiella pneumoniae (34.8%).The study underlines problem of ESBL and MBL mediated resistance, which has created a therapeutic challenge for the clinicians and microbiologists. Simple disk method can be routinely employed to detect these common resistance mechanisms which will reduce the mortality and also spread of such resistant strains.


Keywords: Gram Negative, ESBL, MBL, Antibiotic resistance, Beta lactamases, Infection Control, Antibiotic Policy
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How to cite this article:

Narinder Kaur, Amandeep Kaur and Satnam Singh. 2017. Prevalence of ESBL and MBL Producing Gram Negative Isolates from Various Clinical Samples in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 6(4): 1423-1430. doi: https://doi.org/10.20546/ijcmas.2017.604.174