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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 2907-2915
DOI: https://doi.org/10.20546/ijcmas.2020.912.345


Surface Sterilization Protocol for Nodal and Inter Node Explants of Kankoda (Momordica dioica Roxb.)
S. S. Dodake1, A. S. Jadhav2, S. V. Pawar2 and N. M. Magar2*
1Wheat Specialist, Agricultural Research Station, MPKV, Niphad, India
2State Biotechnology Unit, Department of Agril Botany, PGI, MPKV, Rahuri, India
*Corresponding author
Abstract:

To standardize the sterilization procedure for nodal segment and internodes explants of female Kankoda. Comparison was done between two important sterilizing agents, mercuric chloride (0.1%) and sodium hypochlorite (1%) with four time duration 3, 5, 7 and 10 minutes and in combinations. Result showed that amongst the two sterilants i.e. HgCl2 and NaOCl in combination was found better for controlling the infection. Mercuric chloride (HgCl2) for 5 minute and sodium hypochlorite (NaOCl) for 5 minute in combination was selected for suitable sterilization agents after 10 minute of DDW wash, 10 minute dip in bavistin and at last washed with double distilled water for nodal segments (83.33 % survival) and internodes segments (83.33 % survival) explants of Kankoda. This is the first report of successful sterilization and reduced contamination of explants from naturally field grown Kankoda by using HgCl2 and NaOCl sterilizing agents in combination for standardizing efficient in vitro surface sterilization protocol.


Keywords: Kankoda, Momordica dioica
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How to cite this article:

Dodake, S. S., A. S. Jadhav, S. V. Pawar and Magar, N. M. 2020. Surface Sterilization Protocol for Nodal and Inter Node Explants of Kankoda (Momordica dioica Roxb.).Int.J.Curr.Microbiol.App.Sci. 9(12): 2907-2915. doi: https://doi.org/10.20546/ijcmas.2020.912.345