National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
To standardize the sterilization procedure for nodal segment and internodes explants of female Kankoda. Comparison was done between two important sterilizing agents, mercuric chloride (0.1%) and sodium hypochlorite (1%) with four time duration 3, 5, 7 and 10 minutes and in combinations. Result showed that amongst the two sterilants i.e. HgCl2 and NaOCl in combination was found better for controlling the infection. Mercuric chloride (HgCl2) for 5 minute and sodium hypochlorite (NaOCl) for 5 minute in combination was selected for suitable sterilization agents after 10 minute of DDW wash, 10 minute dip in bavistin and at last washed with double distilled water for nodal segments (83.33 % survival) and internodes segments (83.33 % survival) explants of Kankoda. This is the first report of successful sterilization and reduced contamination of explants from naturally field grown Kankoda by using HgCl2 and NaOCl sterilizing agents in combination for standardizing efficient in vitro surface sterilization protocol.
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