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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 2603-2609
DOI: https://doi.org/10.20546/ijcmas.2020.912.308


Characterization of Alternaria Species Causing Early Blight of Tomato
K.P. Ragupathi1*, P. R. Renganayaki1, S. Sundareswaran1, S.Mohan Kumar2 and A. Kamalakannan3
1Department of Seed Science & Technology, TNAU, Coimbatore, India
2CPMB & B, TNAU, Coimbatore, India
3Department of Plant Pathology, TNAU, Coimbatore, India
*Corresponding author
Abstract:

The present study aims at isolation, identification and characterization of different isolates of Alternaria spp from early blight infected seed samples collected at different regions of Tamilnadu. Four different isolates of Alternaria spp were obtained in pure culture which were designated as isolate PKM-CBE, PKM-DHR, PKM-DKL and PKM - KKR. Significant morphological variations in length and breadth of conidia, numbers of horizontal, vertical and oblique separations were observed in the test isolates. Isolates produce cotton mycelia growth with pigmentation varied from yellow, brown, and grey on potato dextrose agar medium. The PCR amplification of the fungal DNA using universal primers ITS1 and ITS4 and sequencing indicated that, isolates PKM-CBE, PKM-DHR, PKM-DKL were Alternaria solani and isolate PKM – KKR was Alternaria alternata. Pathogenicity test on tomato indicated that both Alternaria spp isolates were virulent.


Keywords: Tomato, Early blight, Alternaria solani, Alternaria alternata
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How to cite this article:

Ragupathi, K. P., P. R. Renganayaki, S. Sundareswaran, S. Mohan Kumar and Kamalakannan, A. 2020. Characterization of Alternaria Species Causing Early Blight of Tomato.Int.J.Curr.Microbiol.App.Sci. 9(12): 2603-2609. doi: https://doi.org/10.20546/ijcmas.2020.912.308