National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study aims at isolation, identification and characterization of different isolates of Alternaria spp from early blight infected seed samples collected at different regions of Tamilnadu. Four different isolates of Alternaria spp were obtained in pure culture which were designated as isolate PKM-CBE, PKM-DHR, PKM-DKL and PKM - KKR. Significant morphological variations in length and breadth of conidia, numbers of horizontal, vertical and oblique separations were observed in the test isolates. Isolates produce cotton mycelia growth with pigmentation varied from yellow, brown, and grey on potato dextrose agar medium. The PCR amplification of the fungal DNA using universal primers ITS1 and ITS4 and sequencing indicated that, isolates PKM-CBE, PKM-DHR, PKM-DKL were Alternaria solani and isolate PKM – KKR was Alternaria alternata. Pathogenicity test on tomato indicated that both Alternaria spp isolates were virulent.
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