National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In vitro Evaluation of Endophytic Bacteria for their efficacy against Chickpea Dry Root Rot Causing Pathogen (Rhizoctonia bataticola (Taub.) Butler)
A survey was conducted during January, 2017 rabi season in five major chickpea growing districts of Andhra Pradesh viz., Kurnool, Y.S.R Kadapa, Anantapuramu, Prakasam and Guntur to study the symptoms of dry root rot under field conditions and to isolate the pathogen. The major symptoms observed are bronzing, yellowing and browning of the leaves, browning and blackening of roots, shriveled roots and chlorotic leaves etc., A total of 22 isolates of R. bataticola were obtained and purified by single hyphal tip method and single sclerotial isolation technique. Pathogenicity of R. bataticola isolates was proved by soil inoculation method. Among the isolates tested maximum disease incidence (100 %) was observed with CRb 9 and it was considered as a most virulent pathogen isolate. A total of 40 antagonistic endophytic bacteria were isolated from healthy roots of different chickpea varieties and the antagonistic efficiency was tested using dual culture technique. Among the isolates CREB 37 showed maximum antifungal efficiency with 74.04 % inhibition against virulent R. bataticola isolate CRb 9.
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