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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 1613-1621
DOI: https://doi.org/10.20546/ijcmas.2020.912.191


Antimicrobial Peptide Magnetic Nanoparticles based Molecular Detection of Bacillus cereus in Milk and Milk Products
S. Rathnapraba* and S. Saranya
Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India
*Corresponding author
Abstract:

Bacillus cereus is major spoilage microorganism in dairy products causing foodborne illness. In recent days, food safety is widely becoming an important public health issue, as foodborne diseases present a global public health problem. The rapid and precise monitoring and detection of foodborne pathogens are some of the most effective ways to control and prevent foodborne illness. In the present study, magnetic nanoparticles (MNPs) were prepared and characterized by particle size analyser, Zeta potential, X-ray diffraction (XRD) spectrophotometer analysis, Fourier Transform Infrared Spectroscopy (FTIR) and UV-visible spectrophotometer analysis. The results of the synthesized nanoparticles had particle size of 128 nm and zeta potential value of -50.8 ±2.2 mV. Crystal size of nanoparticle analysed by XRD was 51.24 nm. The magnetic nanoparticle were coupled with antimicrobial peptide cecropin and was utilized for entrapment of foodborne pathogens from milk and milk products followed by magnetic separation and PCR detection targeting HL gene of B. cereus. Further evaluation of nanoarticle entrapment and PCR, 150 food samples were screened for the presence of B.cereus in milk and milk products, which had improved detection sensitivity with 32% positivity in magnetic nanoparticle mediated entrapment followed by PCR when compared to 19% positivity in direct pelleting and PCR method.


Keywords: PCR detection, Magnetic nanoparticles, Bacillus cereus, Milk and milk products
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How to cite this article:

Rathnapraba, S. and Saranya, S. 2020. Antimicrobial Peptide Magnetic Nanoparticles based Molecular Detection of Bacillus cereus in Milk and Milk Products.Int.J.Curr.Microbiol.App.Sci. 9(12): 1613-1621. doi: https://doi.org/10.20546/ijcmas.2020.912.191