National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A laboratory experiment was conducted in the laboratory of Biotechnology at Dayanand College, Hisar in which seeds of rice cv. MR219were used for regeneration of whole plants. Seeds were soaked overnight in sterilized water for 24 hours to soften and imbibe the seed for recovery of embryos. Seed were dehusked with the forceps and collected in the Petri dish or washed for 3-4 times with sterilized distilled water and again washed for one minute in distilled water. After one minute seeds transfer in 75% ethanol for one minute and again sterilized in 30% sodium hypo chloride for 20 minutes. Finally thoroughly washed in sterile water for at least three times under the aseptic conditions and used as the source of material for explants. Mainly, MS (Murashigeand skoog) media was used in the tissue culture Operation. The pH was adjusted to 5.8.Best results of root generations were obtained by using MS media with IBA. The best results of shoot regeneration were obtained by using MS media with BAP and IAA.
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