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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 1150-1157
DOI: https://doi.org/10.20546/ijcmas.2020.912.139


Effect of Different Concentration of Growth Regulators on in vitro Culture of Rice
Yamini Bhanot* and Vivek Shrivastava
Department of Microbiology, Guru Jambheshwar University of Science and Technology, Hisar, India
*Corresponding author
Abstract:

A laboratory experiment was conducted in the laboratory of Biotechnology at Dayanand College, Hisar in which seeds of rice cv. MR219were used for regeneration of whole plants. Seeds were soaked overnight in sterilized water for 24 hours to soften and imbibe the seed for recovery of embryos. Seed were dehusked with the forceps and collected in the Petri dish or washed for 3-4 times with sterilized distilled water and again washed for one minute in distilled water. After one minute seeds transfer in 75% ethanol for one minute and again sterilized in 30% sodium hypo chloride for 20 minutes. Finally thoroughly washed in sterile water for at least three times under the aseptic conditions and used as the source of material for explants. Mainly, MS (Murashigeand skoog) media was used in the tissue culture Operation. The pH was adjusted to 5.8.Best results of root generations were obtained by using MS media with IBA. The best results of shoot regeneration were obtained by using MS media with BAP and IAA.


Keywords: Oryza sativa, Embryogenic callus, Regeneration, Somaclonal variation, Tissue culture
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How to cite this article:

Yamini Bhanot and Vivek Shrivastava. 2020. Effect of Different Concentration of Growth Regulators on in vitro Culture of Rice.Int.J.Curr.Microbiol.App.Sci. 9(12): 1150-1157. doi: https://doi.org/10.20546/ijcmas.2020.912.139