National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The objective of the present study was to isolate and identify protease producing bacteria from soil and wastewater collected from bakery industry especially halwa manufacturing units in Kollam & Karunagappally region (Kerala), India and detection of protease production on gelatine agar plates. Samples were serially diluted and 0.1 ml was spread on nutrient agar plates at 37oC for48 hours. Total 87 dissimilar colonies were selected and detected protease activity on gelatine agar using 0.8 % mercuric chloride and the activity was observed by the occurrence of clear zone around the isolates. Among the 87 isolates, 27 isolates were showed zone formation around colonies and 8 isolates were selected based on zone diameter and named as, TKMFT8, TKMFT10, TKMFT19 TKMFT22, TKMFT25, TKMFT39, TKMFT53, TKMFT61.The selected were identified using cultural characterization, microscopic observation and biochemical identification using Biomerieux VITEK 2 system as Cedecea davisae, Staphylococcus intermedius, Enterobacter asburiae, Alloiococcus otitis and Proteus mirabilis. These bacterial isolates can be use as microbiological tool for recycling of waste from food processing industries.
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