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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 562-574
DOI: https://doi.org/10.20546/ijcmas.2020.912.068


Isolation and Characterization of Multiple Antibiotic Resistant A. hydrophila causing Mortality of Indian Major Carps in Ganjam District, Odisha
P. Chandravanshi, S. K. Udgata, J. Kumari, B. Sahu* and N. C. Pashupalaka
College of Fisheries (OUAT), Rangailunda, Berhampur, Odisha, India
*Corresponding author
Abstract:

The study was conducted to identify and characterize the etiological agent causing disease and mortality in Indian major carp from fresh water fish farms of Ganjam district in Odisha, India. Diseased fish samples showing symptoms of haemorrhages, red sores, ulcerations, loss of appetite, lethargy, pop-eyes, dropsy and tail or fin rots etc. were collected for the isolation and identification of the pathogenic bacteria using microbiological, biochemical and molecular methods. Out of 65 samples collected, 33 presumptive Aeromonas isolates were obtained, which were then pooled to 12 isolates for further analysis based on their source of isolation. The 16S rRNA gene sequencing and phylogenetic analysis of three isolated strains showed close relationship with Aeromonas hydrophila. In Antibiogram study, all three strains were found resistant against more than three commonly used antibiotics having MAR index ≥ 0.5. This study highlights the involvement of multiple antibiotic resistant Aeromonas hydrophila in the disease outbreak of cultured Indian major carps. The study further affirms the superiority of molecular characterization in identifying Aeromonas hydrophila over microbiological and biochemical methods.


Keywords: Aeromonas hydrophila, Aeromoniasis, Antibiotic resistance, Molecular characterization, Phylogenetic analysis
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How to cite this article:

Chandravanshi, P., S. K. Udgata, J. Kumari, B. Sahu and Pashupalaka, N. C. 2020. Isolation and Characterization of Multiple Antibiotic Resistant A. hydrophila causing Mortality of Indian Major Carps in Ganjam District, Odisha.Int.J.Curr.Microbiol.App.Sci. 9(12): 562-574. doi: https://doi.org/10.20546/ijcmas.2020.912.068