National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
During March 2019 in some of the tomato growing fields of Dharwad it was noticed that tomato plants exhibited malformed flower buds and witches broom symptoms at low incidence level and it was predicted to be big bud phytoplasma infection, therefore, infected and healthy samples were collected for confirmation. Total nucleic acid was extracted by following the CTAB method. Nested PCR was followed using two universal phytoplasma P1/P7 primer set and R16F2n/R16R2n primer set and for which amplification was noticed at 1.8kb and 1.2 kb respectively. The P1/P7 PCR product was further sequenced and BLAST analysis was performed for the sample sequence and it showed that nearly 93 per cent sequence similarity to other phytoplasma sequences in the NCBI database. Thus the present study through molecular analysis confirms the presence of phytoplasma infecting tomato in and around Dharwad.
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