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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 543-548
DOI: https://doi.org/10.20546/ijcmas.2020.912.065


New Report of Tomato Big Bud Phytoplasma Disease in Dharwad, Karnataka
K. Priyanka1* and M. S. Patil2
Department of Plant Pathology, University of Agricultural Sciences, Dharwad, Karnataka, India
*Corresponding author
Abstract:

During March 2019 in some of the tomato growing fields of Dharwad it was noticed that tomato plants exhibited malformed flower buds and witches broom symptoms at low incidence level and it was predicted to be big bud phytoplasma infection, therefore, infected and healthy samples were collected for confirmation. Total nucleic acid was extracted by following the CTAB method. Nested PCR was followed using two universal phytoplasma P1/P7 primer set and R16F2n/R16R2n primer set and for which amplification was noticed at 1.8kb and 1.2 kb respectively. The P1/P7 PCR product was further sequenced and BLAST analysis was performed for the sample sequence and it showed that nearly 93 per cent sequence similarity to other phytoplasma sequences in the NCBI database. Thus the present study through molecular analysis confirms the presence of phytoplasma infecting tomato in and around Dharwad.


Keywords: Tomato Big Bud Phytoplasma Disease
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How to cite this article:

Priyanka, K. and Patil, M. S. 2020. New Report of Tomato Big Bud Phytoplasma Disease in Dharwad, Karnataka.Int.J.Curr.Microbiol.App.Sci. 9(12): 543-548. doi: https://doi.org/10.20546/ijcmas.2020.912.065